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从猪体分离的 2 型猪链球菌孤儿反应调节子 CovR 对其生存力和致病性具有正调控作用。

Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig.

机构信息

Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic University, Wuhan, 430023, China.

Hunan International Scientific and Technological Cooperation Base of Brain Tumor Research, Xiangya Hospital, Central South University, Changsha, China.

出版信息

BMC Vet Res. 2023 Nov 22;19(1):243. doi: 10.1186/s12917-023-03808-9.

DOI:10.1186/s12917-023-03808-9
PMID:37990198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10664645/
Abstract

BACKGROUND

Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen. Orphan response regulator CovR plays crucial regulative functions in the survivability and pathogenicity of S. suis 2. However, research on the CovR in S. suis 2 is limited.

RESULTS

In this study, the regulative functions of CovR in the survivability and pathogenicity were investigated in S. suis 2 isolated from a diseased pig. The deletion of CovR significantly weakened the survivability and pathogenicity of S. suis 2. Compared with the wild-type strain, ΔcovR showed slower growth rates and thinner capsular polysaccharides. Moreover, ΔcovR showed reduced adhesion and invasion to Hep-2 cells as well as anti-phagocytosis and anti-killing ability to 3D4 cells and anti-serum killing ability. In addition, the deletion of CovR significantly reduced the colonisation ability of S. suis 2 in mice. The survival rate of mice infected with ΔcovR was increased by 16.7% compared with that of mice infected with S. suis 2. Further, the deletion of CovR led to dramatic changes in metabolism-related pathways in S. suis 2, five of those, including fructose and mannose metabolism, glycerolipid metabolism, ABC transporters, amino sugar and nucleotide sugar metabolism and phosphotransferase system, were significantly down-regulated.

CONCLUSIONS

Based on the results, CovR plays positive regulative functions in the survivability and pathogenicity of S. suis 2 SC19 strain isolated from a pig.

摘要

背景

猪链球菌血清 2 型（S. suis 2）是一种重要的人畜共患病病原体。孤儿响应调节剂 CovR 在 S. suis 2 的生存能力和致病性中发挥着至关重要的调节作用。然而，目前对 S. suis 2 中的 CovR 的研究还很有限。

结果

本研究在从患病猪中分离的 S. suis 2 中研究了 CovR 在生存能力和致病性方面的调节功能。CovR 的缺失显着削弱了 S. suis 2 的生存能力和致病性。与野生型菌株相比，ΔcovR 的生长速度较慢，荚膜多糖较薄。此外，ΔcovR 对 Hep-2 细胞的粘附和侵袭能力以及对 3D4 细胞的抗吞噬和杀伤能力以及对血清的杀伤能力均降低。此外，CovR 的缺失显着降低了 S. suis 2 在小鼠中的定植能力。与感染 S. suis 2 的小鼠相比，感染ΔcovR 的小鼠的存活率增加了 16.7%。此外，CovR 的缺失导致 S. suis 2 代谢相关途径发生显着变化，其中包括果糖和甘露糖代谢、甘油脂质代谢、ABC 转运蛋白、氨基糖和核苷酸糖代谢以及磷酸转移酶系统，这些途径均显着下调。

结论

基于这些结果，CovR 在从猪中分离的 S. suis 2 SC19 菌株的生存能力和致病性中发挥积极的调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/e29d9f40f4c0/12917_2023_3808_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/943babcd1d5e/12917_2023_3808_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/25f2ae647a1e/12917_2023_3808_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/57f6d9e94023/12917_2023_3808_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/19ead4d16e14/12917_2023_3808_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/048f3de62fc0/12917_2023_3808_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/e29d9f40f4c0/12917_2023_3808_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/943babcd1d5e/12917_2023_3808_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/d1a3bd69fde7/12917_2023_3808_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/25f2ae647a1e/12917_2023_3808_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/57f6d9e94023/12917_2023_3808_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/19ead4d16e14/12917_2023_3808_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/048f3de62fc0/12917_2023_3808_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0b/10664645/e29d9f40f4c0/12917_2023_3808_Fig7_HTML.jpg

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