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二维聚丙烯酰胺凝胶电泳的基础与最新进展

Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis.

作者信息

Magdeldin Sameh, Enany Shymaa, Yoshida Yutaka, Xu Bo, Zhang Ying, Zureena Zam, Lokamani Ilambarthi, Yaoita Eishin, Yamamoto Tadashi

机构信息

Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-dori, Niigata, Japan.

Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

出版信息

Clin Proteomics. 2014 Apr 15;11(1):16. doi: 10.1186/1559-0275-11-16. eCollection 2014.

DOI:10.1186/1559-0275-11-16
PMID:24735559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3996944/
Abstract

Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to simultaneously fractionate, identify, and quantify proteins when coupled with mass spectrometric identification or other immunological tests. Although 2-DE was first introduced more than three decades ago, several challenges and limitations to its utility still exist. This review discusses the principles of 2-DE as well as both recent methodological advances and new applications.

摘要

基于凝胶的蛋白质组学是分离蛋白质复合物最通用的方法之一。在这些方法中，二维聚丙烯酰胺凝胶电泳（2-DE）是一种主要的正交方法，当与质谱鉴定或其他免疫测试结合使用时，它被广泛用于同时分离、鉴定和定量蛋白质。尽管2-DE在三十多年前就首次被引入，但它在实用性方面仍然存在一些挑战和局限性。本文综述了2-DE的原理以及最近的方法进展和新应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/3d0347ad1731/1559-0275-11-16-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/e0c2c0d30a10/1559-0275-11-16-1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/c4d47225f942/1559-0275-11-16-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/3d0347ad1731/1559-0275-11-16-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/e0c2c0d30a10/1559-0275-11-16-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/a187a0c37bca/1559-0275-11-16-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/59c4cd3ef8e4/1559-0275-11-16-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/c4d47225f942/1559-0275-11-16-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/3996944/3d0347ad1731/1559-0275-11-16-5.jpg

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