牛至叶过江藤体外光介导的抗菌活性

Light-mediated antibacterial activity of Lippia origanoides H.B.K. in vitro.

作者信息

Barreto Humberto M, Coelho Bruno R C, Menezes-Silva Suellen M P, Siqueira-Júnior José P, Coutinho Henrique D M, Lemos Izabel Cristina S, Sousa Taciana O, Silva Romézio A C, Medeiros Maria das Graças F, Citó Antonia M G L, Lopes José A D

机构信息

Laboratory of Research in Microbiology, Department of Parasitology and Microbiology, Federal University of Piauí, Teresina (PI), Brazil.

出版信息

Photochem Photobiol Sci. 2014 Dec;13(12):1650-4. doi: 10.1039/c4pp00334a. Epub 2014 Oct 31.

DOI:10.1039/c4pp00334a

PMID:25360993

Abstract

An ethanol extract and different partition fractions obtained from Lippia origanoides H.B.K. were assayed for light-mediated activity against strains of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Identical assays were conducted with and without exposure to UV-A (400-315 nm) light to test for light enhanced activity. The ethanol extract and dichloromethane fraction showed light-mediated activity against the S. aureus strain, but not against the E. coli strain. The dichloromethane fraction was more active than the ethanol extract. Naringenin did not display light-mediated activity against the tested bacteria, indicating that the light-mediated antimicrobial activity of the dichloromethane fraction is not due to its major component. The results represent the first report of light-mediated antimicrobial activity of Lippia origanoides and show that its phytochemicals could be used as light-mediated antimicrobial agents.

摘要

对从牛至叶过江藤（Lippia origanoides H.B.K.）中获得的乙醇提取物和不同的分离组分，测定了其对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）菌株的光介导活性。在有和没有暴露于UV-A（400 - 315 nm）光的情况下进行了相同的测定，以测试光增强活性。乙醇提取物和二氯甲烷组分对金黄色葡萄球菌菌株显示出光介导活性，但对大肠杆菌菌株没有。二氯甲烷组分比乙醇提取物更具活性。柚皮素对测试细菌没有显示出光介导活性，表明二氯甲烷组分的光介导抗菌活性不是由于其主要成分。这些结果代表了牛至叶过江藤光介导抗菌活性的首次报道，并表明其植物化学物质可作为光介导抗菌剂。