Kempen Paul J, Hitzman Chuck, Sasportas Laura S, Gambhir Sanjiv S, Sinclair Robert
Department of Material Science and Engineering, Stanford University, 496 Lomita Mall, Stanford, CA 94305-4034 U.S.A.
Stanford Nanocharacterization Laboratory, Stanford University, 476 Lomita Mall, Stanford, CA 94305-4035 U.S.A.
Mater Res Soc Symp Proc. 2013 May 13;1569:157-163. doi: 10.1557/opl.2013.613.
The ability of nano secondary ion mass spectrometry (NanoSIMS) to locate and analyze Raman active gold core nanoparticles (R-AuNPs) in a biological system is compared with the standard analysis using the scanning electron microscope (SEM). The same cell with R-AuNPs on and inside the macrophage was analyzed with both techniques to directly compare them. SEM analysis showed a large number of nanoparticles within the cell. Subsequent NanoSIMS analysis showed fewer R-AuNPs with lower spatial resolution. SEM was determined to be superior to NanoSIMS for the analysis of inorganic nanoparticles in complex biological systems.
将纳米二次离子质谱仪（NanoSIMS）在生物系统中定位和分析拉曼活性金核纳米颗粒（R-AuNPs）的能力与使用扫描电子显微镜（SEM）的标准分析方法进行了比较。用这两种技术对巨噬细胞上和内部含有R-AuNPs的同一细胞进行分析，以直接比较它们。SEM分析显示细胞内有大量纳米颗粒。随后的NanoSIMS分析显示R-AuNPs数量较少且空间分辨率较低。结果表明，在复杂生物系统中分析无机纳米颗粒时，SEM优于NanoSIMS。
