Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, California, USA.
Nat Med. 2012 Apr 15;18(5):829-34. doi: 10.1038/nm.2721.
The difficulty in delineating brain tumor margins is a major obstacle in the path toward better outcomes for patients with brain tumors. Current imaging methods are often limited by inadequate sensitivity, specificity and spatial resolution. Here we show that a unique triple-modality magnetic resonance imaging-photoacoustic imaging-Raman imaging nanoparticle (termed here MPR nanoparticle) can accurately help delineate the margins of brain tumors in living mice both preoperatively and intraoperatively. The MPRs were detected by all three modalities with at least a picomolar sensitivity both in vitro and in living mice. Intravenous injection of MPRs into glioblastoma-bearing mice led to MPR accumulation and retention by the tumors, with no MPR accumulation in the surrounding healthy tissue, allowing for a noninvasive tumor delineation using all three modalities through the intact skull. Raman imaging allowed for guidance of intraoperative tumor resection, and a histological correlation validated that Raman imaging was accurately delineating the brain tumor margins. This new triple-modality-nanoparticle approach has promise for enabling more accurate brain tumor imaging and resection.
脑肿瘤边界的界定困难是提高脑肿瘤患者治疗效果的主要障碍。目前的成像方法通常受到灵敏度、特异性和空间分辨率不足的限制。在这里，我们展示了一种独特的三模态磁共振成像-光声成像-拉曼成像纳米粒子（称为 MPR 纳米粒子），它可以在活体小鼠中准确地帮助术前和术中界定脑肿瘤的边界。在体外和活体小鼠中，MPR 都可以通过所有三种模态进行检测，其灵敏度至少达到皮摩尔级。将 MPR 注入胶质母细胞瘤荷瘤小鼠体内后，MPR 会在肿瘤中积累和保留，而周围健康组织中没有 MPR 积累，从而可以通过完整的颅骨使用所有三种模态进行无创肿瘤界定。拉曼成像可以指导术中肿瘤切除，组织学相关性验证了拉曼成像可以准确地界定脑肿瘤边界。这种新的三模态纳米粒子方法有望实现更精确的脑肿瘤成像和切除。
