Kaka A, Wahid H, Rosnina Y, Yimer N, Khumran A M, Behan A A, Ebrahimi M
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia; Sindh Agriculture University, Tando jam, Pakistan.
Reprod Domest Anim. 2015 Feb;50(1):29-33. doi: 10.1111/rda.12445. Epub 2014 Nov 3.
The study was conducted to evaluate the effects of α-linolenic acid (ALA) on frozen-thawed quality and fatty acid composition of bull sperm. For that, twenty-four ejaculates obtained from three bulls were diluted in a Tris extender containing 0 (control), 3, 5, 10 and 15 ng/ml of ALA. Extended semen was incubated at 37°C for 15 min, to allow absorption of ALA by sperm cell membrane. The sample was chilled for 2 h, packed into 0.25-ml straws and frozen in liquid nitrogen for 24 h. Subsequently, straws were thawed and evaluated for total sperm motility (computer-assisted semen analysis), membrane functional integrity (hypo-osmotic swelling test), viability (eosin-nigrosin), fatty acid composition (gas chromatography) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)). A higher (p < 0.05) percentage of total sperm motility was observed in ALA groups 5 ng/ml (47.74 ± 07) and 10 ng/ml (44.90 ± 0.7) in comparison with control (34.53 ± 3.0), 3 ng/ml (34.40 ± 2.6) and 15 ng/ml (34.60 ± 2.9). Still, the 5 ng/ml ALA group presented a higher (p < 0.05) percentage of viable sperms (74.13 ± 0.8) and sperms with intact membrane (74.46 ± 09) than all other experimental groups. ALA concentration and lipid peroxidation in post-thawed sperm was higher in all treated groups when compared to the control group. As such, the addition of 5 ng/ml of ALA to Tris extender improved quality of frozen-thawed bull spermatozoa.
本研究旨在评估α-亚麻酸（ALA）对公牛精子冻融质量和脂肪酸组成的影响。为此，从三头公牛采集的24份精液样本，分别用含有0（对照）、3、5、10和15 ng/ml ALA的Tris稀释液进行稀释。稀释后的精液在37°C下孵育15分钟，以使精子细胞膜吸收ALA。样本冷却2小时后，装入0.25 ml的细管中，并在液氮中冷冻24小时。随后，将细管解冻，并对精子的总活力（计算机辅助精液分析）、膜功能完整性（低渗肿胀试验）、活力（伊红-黑色素染色）、脂肪酸组成（气相色谱法）和脂质过氧化（硫代巴比妥酸反应物质（TBARS））进行评估。与对照组（34.53±3.0）、3 ng/ml组（34.40±2.6）和15 ng/ml组（34.60±2.9）相比，5 ng/ml（47.74±0.7）和10 ng/ml（44.90±0.7）的ALA组观察到更高（p<0.05）的总精子活力百分比。此外，5 ng/ml ALA组的活精子（74.13±0.8）和膜完整精子（74.46±0.9）百分比高于所有其他实验组。与对照组相比，所有处理组解冻后精子中的ALA浓度和脂质过氧化水平更高。因此，在Tris稀释液中添加5 ng/ml的ALA可提高冻融后公牛精子的质量。
