Kaka Asmatullah, Wahid Haron, Rosnina Yusoff, Yimer Nurhusien, Khumran A M, Sarsaifi Kazhal, Behan Atique Ahmed, Kaka Ubedullah, Ebrahimi M
Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Sindh Agriculture University, Tando Jam, Pakistan.
Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
Anim Reprod Sci. 2015 Feb;153:1-7. doi: 10.1016/j.anireprosci.2014.12.001. Epub 2014 Dec 10.
The present study was conducted to determine the effects of supplementing α-linolenic acid (ALA) into BioXcell(®) extender on post-cooling, post-thawed bovine spermatozoa and post thawed fatty acid composition. Twenty-four semen samples were collected from three bulls using an electro-ejaculator. Fresh semen samples were evaluated for general motility using computer assisted semen analyzer (CASA) whereas morphology and viability with eosin-nigrosin stain. Semen samples extended into BioXcell(®) were divided into five groups to which 0, 3, 5, 10 and 15 ng/ml of ALA were added, respectively. The treated samples were incubated at 37°C for 15 min for ALA uptake by sperm cells before being cooled for 2 h at 5°C. After evaluation, the cooled samples were packed into 0.25 ml straws and frozen in liquid nitrogen for 24 h before thawing and evaluation for semen quality. Evaluation of cooled and frozen-thawed semen showed that the percentages of all the sperm parameters improved with 5 ng/ml ALA supplement. ALA was higher in all treated groups than control groups than control group. In conclusion, 5 ng/ml ALA supplemented into BioXcell(®) extender improved the cooled and frozen-thawed quality of bull spermatozoa.
本研究旨在确定在BioXcell®稀释液中添加α-亚麻酸（ALA）对牛精子冷休克后、解冻后以及解冻后脂肪酸组成的影响。使用电刺激采精器从三头公牛采集了24份精液样本。使用计算机辅助精液分析仪（CASA）评估新鲜精液样本的总体活力，而使用伊红-黑色素染色评估形态和活力。将稀释到BioXcell®中的精液样本分为五组，分别添加0、3、5、10和15 ng/ml的ALA。处理后的样本在37°C孵育15分钟以使精子细胞摄取ALA，然后在5°C冷却2小时。评估后，将冷却后的样本装入0.25 ml细管中，在液氮中冷冻24小时，然后解冻并评估精液质量。对冷却和冻融精液的评估表明，添加5 ng/ml ALA可提高所有精子参数的百分比。所有处理组中的ALA含量均高于对照组。总之，在BioXcell®稀释液中添加5 ng/ml ALA可提高公牛精子的冷却和冻融质量。
