Dalgaard P, Ross T, Kamperman L, Neumeyer K, McMeekin T A
Technological Laboratory, Ministry of Fisheries, Technical University, Lyngby, Denmark.
Int J Food Microbiol. 1994 Nov;23(3-4):391-404. doi: 10.1016/0168-1605(94)90165-1.
The relationship between maximum specific growth rates (mu max) determined from viable counts and turbidimetric measurements for a range of bacterial species is examined in order to assess the potential of turbidimetric methods in predictive microbiology. Two methods for the estimation of mu max from turbidimetric data are presented. One is based on absorbance and the other on transmittance measurements. Both are compared to estimates obtained by viable count methods. Calibration factors, a function to correct the non-linearity of absorbance measurements, and variance stabilising transformations for corrected absorbance measurements and for viable count data, are determined. It is concluded that turbidimetric measurements may be used reliably for estimation of mu max.
为了评估比浊法在预测微生物学中的潜力，研究了一系列细菌物种通过活菌计数法和比浊法测量得出的最大比生长速率（μmax）之间的关系。提出了两种从比浊数据估算μmax的方法。一种基于吸光度，另一种基于透光率测量。将这两种方法与通过活菌计数法获得的估算值进行比较。确定了校准因子、校正吸光度测量非线性的函数以及校正吸光度测量和活菌计数数据的方差稳定变换。得出的结论是，比浊测量可可靠地用于估算μmax。
