Zhang S, Guo Y, Zou H, Sun N, Zhao D, Liu W, Dong Y, Cheng G, Yuan Q
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, 14 Third Section, Renmin Nan Road, 610041, Chengdu, China.
Osteoporos Int. 2015 Mar;26(3):1073-80. doi: 10.1007/s00198-014-2952-6. Epub 2014 Nov 1.
We established a chronic kidney disease (CKD) mouse model with estrogen deficiency and inserted titanium implants into the femur of such mice to investigate the fixation of the implants. Both the histomorphometry and implant resistance indicated that estrogen deficiency impaired the fixation of titanium implants inserted into such mice.
CKD has been regarded as a worldwide public health problem. Estrogen is a critical factor for both renal protection and bone remodeling. A previous study demonstrated that CKD impairs the early healing of titanium implants. However, the combined effect of estrogen deficiency and CKD on the fixation of titanium implants is largely unknown.
Forty 9-week-old female C57BL mice were randomly divided into sham, ovariectomy (OVX), CKD, and CKD + OVX groups. Uremia and estrogen deficiency were induced by 5/6 nephrectomy and OVX, respectively. Experimental titanium implants were inserted into the distal end of the femur. Bone-implant contact (BIC) ratio and bone volume (BV/TV) around the implants were histomorphometrically analyzed. The fixation strength of the implant was measured by a biomechanical push-in resistance test.
Serum measurement confirmed a significant increase in serum blood urea nitrogen (BUN) in the CKD group, which was further increased by OVX. Estrogen deficiency led to significant decreases in the BIC ratio, BV/TV, and the push-in resistance in CKD animals. There was a significant interaction between the effects of OVX and CKD, with OVX exacerbating the effects of CKD on BIC ratio and push-in resistance.
The results indicated that estrogen deficiency exerts a synergistic effect with CKD and further impairs the fixation of titanium implants in CKD mice.
我们建立了雌激素缺乏的慢性肾脏病（CKD）小鼠模型，并将钛植入物植入此类小鼠的股骨中，以研究植入物的固定情况。组织形态计量学和植入物抗力均表明，雌激素缺乏会损害植入此类小鼠体内的钛植入物的固定。
CKD已被视为一个全球性的公共卫生问题。雌激素是肾脏保护和骨重塑的关键因素。先前的一项研究表明，CKD会损害钛植入物的早期愈合。然而，雌激素缺乏和CKD对钛植入物固定的联合作用在很大程度上尚不清楚。
将40只9周龄雌性C57BL小鼠随机分为假手术组、卵巢切除术（OVX）组、CKD组和CKD + OVX组。分别通过5/6肾切除术和OVX诱导尿毒症和雌激素缺乏。将实验性钛植入物插入股骨远端。通过组织形态计量学分析植入物周围的骨-植入物接触（BIC）率和骨体积（BV/TV）。通过生物力学推入抗力试验测量植入物的固定强度。
血清检测证实CKD组血清血尿素氮（BUN）显著升高，OVX进一步使其升高。雌激素缺乏导致CKD动物的BIC率、BV/TV和推入抗力显著降低。OVX和CKD的作用之间存在显著交互作用，OVX加剧了CKD对BIC率和推入抗力的影响。
结果表明，雌激素缺乏与CKD具有协同作用，进一步损害了CKD小鼠体内钛植入物的固定。
