Romanyuk Andrey V, Zvezdin Vasiliy N, Samant Pradnya, Grenader Mark I, Zemlyanova Marina, Prausnitz Mark R
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology , 311 Ferst Drive, Atlanta, Georgia 30332 United States.
Anal Chem. 2014 Nov 4;86(21):10520-3. doi: 10.1021/ac503823p. Epub 2014 Oct 17.
Clinical medicine and public health would benefit from simplified acquisition of biological samples from patients that can be easily obtained at point of care, in the field, and by patients themselves. Microneedle patches are designed to serve this need by collecting dermal interstitial fluid containing biomarkers without the dangers, pain, or expertise needed to collect blood. This study presents novel methods to collect biomarker analytes from microneedle patches for analysis by integration into conventional analytical laboratory microtubes and microplates. Microneedle patches were made out of cross-linked hydrogel composed of poly(methyl vinyl ether-alt-maleic acid) and poly(ethylene glycol) prepared by micromolding. Microneedle patches were shown to swell with water up to 50-fold in volume, depending on degree of polymer cross-linking, and to collect interstitial fluid from the skin of rats. To collect analytes from microneedle patches, the patches were mounted within the cap of microcentrifuge tubes or formed the top of V-bottom multiwell microplates, and fluid was collected in the bottom of the tubes under gentle centrifugation. In another method, microneedle patches were attached to form the bottom of multiwell microplates, thereby enabling in situ analysis. The simplicity of biological sample acquisition using microneedle patches coupled with the simplicity of analyte collection from microneedles patches integrated into conventional analytical equipment could broaden the reach of future screening, diagnosis, and monitoring of biomarkers in healthcare and environmental/workplace settings.
临床医学和公共卫生将受益于从患者那里简化获取生物样本的方式，这些样本可以在护理点、现场轻松获取，甚至患者自己也能获取。微针贴片旨在满足这一需求，通过收集含有生物标志物的真皮间质液，而无需承担采集血液所需面临的风险、疼痛或专业技能。本研究提出了从微针贴片中收集生物标志物分析物的新方法，即将其整合到传统分析实验室的微量管和微孔板中进行分析。微针贴片由通过微成型制备的聚（甲基乙烯基醚-alt-马来酸）和聚（乙二醇）组成的交联水凝胶制成。微针贴片根据聚合物交联程度，在水中体积可膨胀至50倍，并能从大鼠皮肤收集间质液。为了从微针贴片中收集分析物，将贴片安装在微量离心管的管帽内或形成V型底多孔微孔板的顶部，在轻轻离心的情况下，液体收集在管底。在另一种方法中，微针贴片附着形成多孔微孔板的底部，从而实现原位分析。使用微针贴片获取生物样本的简便性，以及将微针贴片与集成到传统分析设备中收集分析物的简便性相结合，可能会拓宽未来在医疗保健以及环境/工作场所环境中对生物标志物进行筛查、诊断和监测的范围。
