Hu Zhixiong, Glidle Andrew, Ironside Charles, Cooper Jonathan M, Yin Huabing
Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow, UK.
Lab Chip. 2015 Jan 7;15(1):283-9. doi: 10.1039/c4lc00952e.
We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel "bead-AWG" platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.
我们描述了一种用于局部复用荧光测量的集成透镜阵列波导光栅（AWG）微型光谱仪的开发。该设备占地面积仅1毫米宽、1厘米长，能够在芯片上进行光谱测量。基于来自电荷耦合器件（CCD）相机的简单强度读数，可同时测量多个荧光信号。我们还展示了AWG光谱仪与微流体平台的集成，利用透镜功能限制光束形状以实现聚焦照明。这种能力增强了信号收集，提供了更好的空间分辨率，并为分析流动中的小体积样品（如细胞）提供了途径。为展示这些能力，我们开发了一种新型“珠子-AWG”平台，利用该平台我们展示了同时或相继的局部复用荧光检测。这样一个集成系统为能够从单个定义位置进行多波长荧光光学检测的便携式系统奠定了基础。
