Irawan Rudi, Tjin Swee Chuan, Yager Paul, Zhang Dianwen
Biomedical Engineering Research Centre, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 637553.
Biomed Microdevices. 2005 Sep;7(3):205-11. doi: 10.1007/s10544-005-3027-4.
Development of a compact fluorescence-based detection system for use in a micro-analytical system, such as a point-of-care diagnostic system, often requires a multi-channel microfluidic chip system. Since the materials used for microfluidic chips usually are transparent in the visible region and have a refractive indices higher than that of air or the surrounding environment, the fluorescence emission and scattered excitation light can propagate through the chip. We observed that such propagation can cause cross-talk between adjacent channels, and may become the major source of noise in the system and/or photo bleach the fluorescent samples in the adjacent channels, particularly for the small distances between the channels found in microfluidic chips, usually in order of several micro m. We monitored this cross-talk using fluorescein as a fluorescent sample and Mylar sheeting as a microfluidic chip material. We then discuss how this cross-talk can be avoided using a simple, inexpensive and effective method.
开发用于微分析系统（如即时诊断系统）的紧凑型荧光检测系统通常需要多通道微流控芯片系统。由于用于微流控芯片的材料在可见光区域通常是透明的，并且其折射率高于空气或周围环境的折射率，荧光发射和散射的激发光可以通过芯片传播。我们观察到这种传播会导致相邻通道之间的串扰，并可能成为系统中的主要噪声源和/或使相邻通道中的荧光样品发生光漂白，特别是对于微流控芯片中通道之间通常为几微米量级的小距离。我们使用荧光素作为荧光样品，聚酯薄膜作为微流控芯片材料来监测这种串扰。然后我们讨论如何使用一种简单、廉价且有效的方法来避免这种串扰。
