铜绿假单胞菌的耐药基因组与表型易感性的关系
The resistome of Pseudomonas aeruginosa in relationship to phenotypic susceptibility.
作者信息
Kos Veronica N, Déraspe Maxime, McLaughlin Robert E, Whiteaker James D, Roy Paul H, Alm Richard A, Corbeil Jacques, Gardner Humphrey
机构信息
Infection Innovative Medicines Unit, AstraZeneca R&D Boston, Waltham, Massachusetts, USA
Infectious Diseases Research Center, Laval University, Quebec, Quebec, Canada.
出版信息
Antimicrob Agents Chemother. 2015 Jan;59(1):427-36. doi: 10.1128/AAC.03954-14. Epub 2014 Nov 3.
Many clinical isolates of Pseudomonas aeruginosa cause infections that are difficult to eradicate due to their resistance to a wide variety of antibiotics. Key genetic determinants of resistance were identified through genome sequences of 390 clinical isolates of P. aeruginosa, obtained from diverse geographic locations collected between 2003 and 2012 and were related to microbiological susceptibility data for meropenem, levofloxacin, and amikacin. β-Lactamases and integron cassette arrangements were enriched in the established multidrug-resistant lineages of sequence types ST111 (predominantly O12) and ST235 (O11). This study demonstrates the utility of next-generation sequencing (NGS) in defining relevant resistance elements and highlights the diversity of resistance determinants within P. aeruginosa. This information is valuable in furthering the design of diagnostics and therapeutics for the treatment of P. aeruginosa infections.
许多铜绿假单胞菌临床分离株会引发难以根除的感染，因为它们对多种抗生素具有耐药性。通过对390株铜绿假单胞菌临床分离株的基因组序列进行分析，确定了耐药性的关键遗传决定因素。这些分离株于2003年至2012年间从不同地理位置收集，并且与美罗培南、左氧氟沙星和阿米卡星的微生物药敏数据相关。β-内酰胺酶和整合子盒排列在序列类型ST111（主要为O12）和ST235（O11）的既定多重耐药谱系中富集。本研究证明了下一代测序（NGS）在确定相关耐药元件方面的实用性，并突出了铜绿假单胞菌耐药决定因素的多样性。这些信息对于进一步设计治疗铜绿假单胞菌感染的诊断方法和治疗手段具有重要价值。
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