Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, P.O. Box 2040, 3000 CA Rotterdam, the Netherlands.
Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, P.O. Box 2040, 3000 CA Rotterdam, the Netherlands.
Int J Antimicrob Agents. 2018 Sep;52(3):407-410. doi: 10.1016/j.ijantimicag.2018.05.009. Epub 2018 May 15.
Pseudomonas aeruginosa is one of the most important causes of infection in intensive care units (ICUs). It is intrinsically resistant to many antimicrobials and easily acquires additional resistance genes via horizontal gene transfer of mobile genetic elements. In this study, 1528 P. aeruginosa isolates obtained from a Dutch national surveillance programme between the years 1998-2011 were analysed for the presence of extended-spectrum β-lactamase (ESBL) genes (bla, bla, bla, bla, bla, bla and bla) and metallo-β-lactamase (MBL) genes (bla, bla and bla). Of the ceftazidime-resistant isolates, 6.2% tested phenotypically positive for ESBL. Moreover, a Verona integron-encoded MBL (VIM) gene was found in 3.1% of isolates that were phenotypically resistant to imipenem and/or meropenem. Multilocus sequence typing (MLST) of ESBL-positive isolates indicated ST1216, ST111 and ST622, with all bla-positive isolates belonging to the ST111 clone. Although the prevalence of ESBL and MBL phenotypes in this Dutch national surveillance collection of >1500 ICU P. aeruginosa isolates was very low, all VIM-producing isolates belonged to the high risk-associated, international, clonal complex CC111, and most ESBL-producing isolates belonged to clonal complexes known for their successful spread, e.g. CC111 and CC235. These data indicate that high-risk clones of P. aeruginosa were present in the Netherlands between 1998-2011 and probably spread unnoticed throughout Dutch hospitals.
铜绿假单胞菌是重症监护病房（ICU）感染的最重要原因之一。它对许多抗菌素有固有耐药性，并且很容易通过移动遗传元件的水平基因转移获得额外的耐药基因。在这项研究中，分析了 1998 年至 2011 年间从荷兰国家监测计划中获得的 1528 株铜绿假单胞菌分离株，以检测是否存在超广谱β-内酰胺酶（ESBL）基因（bla、bla、bla、bla、bla、bla 和 bla）和金属β-内酰胺酶（MBL）基因（bla、bla 和 bla）。在头孢他啶耐药的分离株中，6.2%表现出表型阳性的 ESBL。此外，在对亚胺培南和/或美罗培南表型耐药的 3.1%的分离株中发现了一种 Verona 整合子编码的 MBL（VIM）基因。ESBL 阳性分离株的多位点序列分型（MLST）表明 ST1216、ST111 和 ST622，所有 bla 阳性分离株均属于 ST111 克隆。尽管在这项荷兰国家 ICU 铜绿假单胞菌分离株监测收集的超过 1500 株中，ESBL 和 MBL 表型的流行率非常低，但所有产生 VIM 的分离株均属于高风险相关的国际克隆复合体 CC111，大多数产生 ESBL 的分离株属于因其成功传播而闻名的克隆复合体，例如 CC111 和 CC235。这些数据表明，1998-2011 年间，高风险铜绿假单胞菌克隆存在于荷兰，并且可能在荷兰医院中未被察觉地传播。
