Malinin G I, Hornicek F J, Malinin T I
Department of Physics, Georgetown University, Washington, DC 20057.
Biochem Biophys Res Commun. 1989 Jan 31;158(2):590-4. doi: 10.1016/s0006-291x(89)80090-7.
Increased rates of protein phosphorylation, IL-2 receptor (IL-2R) expression, IL-2 production and DNA synthesis were quantified in rat lymphocytes oxidized by periodic acid (H5IO6). Enhanced phosphorylation of 97, 59 and 37 to 29 kDa proteins in lymphocytes was detected at 18 and 36 hrs post-oxidation, whereas IL-2R expression and IL-2 elaboration were at their maximum by 24 hrs. The number of oxidized cells entering the S-phase of the cell cycle and their thymidine incorporation rates reached their maximum at 72 hrs. These results indicate that H5IO6-induced blastogenesis elicits a progression of responses temporally similar to those of lymphocytes stimulated by lectins.
对经高碘酸（H5IO6）氧化的大鼠淋巴细胞中蛋白质磷酸化、白细胞介素-2受体（IL-2R）表达、白细胞介素-2产生和DNA合成速率的增加进行了定量分析。在氧化后18小时和36小时检测到淋巴细胞中97 kDa、59 kDa以及37至29 kDa蛋白质的磷酸化增强，而IL-2R表达和IL-2分泌在24小时时达到最大值。进入细胞周期S期的氧化细胞数量及其胸苷掺入率在72小时时达到最大值。这些结果表明，H5IO6诱导的细胞增殖引发了一系列反应，其时间进程与凝集素刺激的淋巴细胞类似。
