Malinin G I, Hornicek F J, Malinin T I
Department of Physics, Georgetown University, Washington, DC 20057.
Biosci Rep. 1990 Aug;10(4):347-52. doi: 10.1007/BF01117234.
Blastogenic and cytotoxic effects of hexavalent chromium were evaluated by direct, 2 and 20 min oxidation of lymphocytes by 10.0 to 0.0005 mM CrO3 at 0 degrees C. Oxidized cells exhibited concentration-dependent cytotoxicity and the inhibition of tritiated thymidine incorporation rates. When lymphocytes were oxidized first by 1.0 mM periodic acid (H5IO6) and thereafter by 1.0 mM CrO3, the viability and [3H]-TdR incorporation rates of sequentially oxidized cells were identical to the corresponding indicators of lymphocytes oxidized only by CrO3. The reversal of the oxidation sequence restored [3H]-TdR incorporation to control levels and increased cell survival. It is therefore concluded that direct interaction of hexavalent CrO3 with plasma membrane of lymphocytes results in concentration-dependent cytotoxicity and the inhibition of [3H]-TdR incorporation, but it does not induce blastogenesis.
通过在0℃下用10.0至0.0005 mM三氧化铬对淋巴细胞进行直接、2分钟和20分钟的氧化，评估六价铬的致有丝分裂和细胞毒性作用。氧化后的细胞表现出浓度依赖性细胞毒性以及氚化胸腺嘧啶核苷掺入率的抑制。当淋巴细胞先用1.0 mM高碘酸（H5IO6）氧化，然后用1.0 mM三氧化铬氧化时，顺序氧化细胞的活力和[3H]-TdR掺入率与仅用三氧化铬氧化的淋巴细胞的相应指标相同。氧化顺序的颠倒使[3H]-TdR掺入恢复到对照水平并提高了细胞存活率。因此得出结论，六价三氧化铬与淋巴细胞质膜的直接相互作用导致浓度依赖性细胞毒性和[3H]-TdR掺入的抑制，但不会诱导细胞增殖。
