从新生儿重症监护病房食用的婴儿配方奶粉中分离出的普氏塔特姆菌菌株的分离、鉴定及药敏模式：来自伊朗的首次报告
Isolation, Identification and Antimicrobial Susceptibility Pattern of Tatumella ptyseos Strains Isolated From Powdered Infant Formula Milk Consumed in Neonatal Intensive Care Unit: First Report From Iran.
作者信息
Mardaneh Jalal, Soltan Dallal Mohammad Mehdi, Taheripoor Mehrnaz, Rajabi Zahra
机构信息
Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran.
Division of Bacteriology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran ; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, IR Iran.
出版信息
Jundishapur J Microbiol. 2014 Jun;7(6):e10608. doi: 10.5812/jjm.10608. Epub 2014 Jun 1.
BACKGROUND
Tatumella ptyseos is a rod-shaped, Gram-negative, facultative, and anaerobic bacteria categorized in the Enterobacteriaceae family. It is a rare food-borne opportunistic pathogen which causes neonatal sepsis, bacteremia, and urinary tract infections. T. ptyseos has been also cultured from various food sources around the world.
OBJECTIVES
It is difficult to determine the source of the infection in the patients (especially newborns) due to low information about the epidemiology of T. ptyseos. The current study aimed to investigate the isolation, identification and antimicrobial susceptibility pattern of T. ptyseos strains from the consumed powdered infant formula milk (PIF) in hospital neonatal intensive care unit (NICU).
MATERIALS AND METHODS
A total of 125 powdered infant formula milk (PIF) samples were purchased from drug stores from June 2011 to March 2012. T. ptyseos was isolated according to food and drug administration (FDA) method. For final confirmation, biochemical testes embedded in API-20E system were used. Drug susceptibility test was performed using the disc diffusion method, according to clinical and laboratory standard institute (CLSI) recommendations.
RESULTS
Results of the study showed that, out of 125 samples, T. ptyseos was isolated from four (3/2%) PIF samples. All isolated strains (100%) were resistant to ampicillin, carbenicillin, cotrimoxazole and amoxicillin.
CONCLUSIONS
The present study was the first report on the isolation and identification of T. ptyseos from PIF in Iran. T. ptyseos are frequently present in various kinds of foods; therefore, further investigation on these samples is required. It is necessary to track the T. ptyseos in a wide variety of foods and individuals especially in immunocompromised people such as human immunodeficiency virus (HIV)-positive patients to reveal the possible routes of transmission of this pathogen to humans. In addition, molecular studies are required to determine the genetic relationship between T. ptyseos strains isolated from different sources.
背景
普氏塔特姆菌是一种杆状、革兰氏阴性、兼性厌氧细菌，属于肠杆菌科。它是一种罕见的食源性机会致病菌，可导致新生儿败血症、菌血症和尿路感染。世界各地的各种食物来源中也培养出了普氏塔特姆菌。
目的
由于关于普氏塔特姆菌流行病学的信息较少，难以确定患者（尤其是新生儿）的感染源。本研究旨在调查医院新生儿重症监护病房（NICU）食用的婴儿配方奶粉（PIF）中普氏塔特姆菌菌株的分离、鉴定及抗菌药敏模式。
材料与方法
2011年6月至2012年3月，从药店购买了共125份婴儿配方奶粉（PIF）样本。根据食品药品监督管理局（FDA）方法分离普氏塔特姆菌。为进行最终确认，使用了API-20E系统中的生化试验。根据临床和实验室标准协会（CLSI）的建议，采用纸片扩散法进行药敏试验。
结果
研究结果显示，在125份样本中，从4份（3.2%）PIF样本中分离出了普氏塔特姆菌。所有分离菌株（100%）对氨苄西林、羧苄西林、复方新诺明和阿莫西林耐药。
结论
本研究是伊朗首次关于从PIF中分离和鉴定普氏塔特姆菌的报告。普氏塔特姆菌经常存在于各种食物中；因此，需要对这些样本进行进一步调查。有必要在各种食物和个体中追踪普氏塔特姆菌，尤其是在免疫功能低下的人群中，如人类免疫缺陷病毒（HIV）阳性患者，以揭示该病原体向人类传播的可能途径。此外，需要进行分子研究以确定从不同来源分离的普氏塔特姆菌菌株之间的遗传关系。
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