Llanco Luis A, Nakano Viviane, Avila-Campos Mario J
Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Brazil.
Curr Microbiol. 2015 Mar;70(3):330-7. doi: 10.1007/s00284-014-0722-5. Epub 2014 Nov 6.
The sialidase activity and genetic diversity of 22 Clostridium perfringens strains isolated from chickens with necrotic enteritis were determined. Sialidase activity was detected in 86.4 % of the strains. All C. perfringens showed a high value of similarity (>96 %), and they were grouped into seven clusters clearly separated from the other reference bacterial strains. From these clusters four patterns were defined in accordance with their phenotypic (sialidase production and antibiotic resistance profile) and genotypic (presence of nanI and nanJ genes) characteristics. Our results showed heterogeneity among strains, but they were genotypically similar, and it is suggested further studies are needed to better understand the pathogenesis of necrotic enteritis.
对从患有坏死性肠炎的鸡中分离出的22株产气荚膜梭菌菌株的唾液酸酶活性和遗传多样性进行了测定。在86.4%的菌株中检测到唾液酸酶活性。所有产气荚膜梭菌均显示出较高的相似性值（>96%），并被分为七个簇，与其他参考细菌菌株明显分开。根据其表型（唾液酸酶产生和抗生素耐药谱）和基因型（nanI和nanJ基因的存在）特征，从这些簇中定义了四种模式。我们的结果显示菌株之间存在异质性，但它们在基因型上相似，建议需要进一步研究以更好地了解坏死性肠炎的发病机制。
