Xing Siyi, Zhang Mengran, Yan Dujian, Tian Tian, Yu Aohang, Wu Chenchen
College of Animal Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
AKS Vocational and Technical College, Aksu, 830000, Xinjiang, China.
Vet Res Commun. 2025 Aug 26;49(5):289. doi: 10.1007/s11259-025-10856-z.
Clostridium perfringens (Cp) type A is one of the primary microorganisms responsible for bacterial diarrhoea in weaning piglets. Matrine, a tetracyclic quinolizidine alkaloid, exhibits diverse bioactive properties, including anti-inflammatory and neuroprotective effects. To better understand the anti-inflammatory mechanisms of Matrine on piglets infected with Cp type A (CpA). We investigated its preventive effects against diarrhoea over a 14-days period. Our results showed that 30 mg/kg of Matrine is non-toxic and does not harm the histological structure of organ tissues or alter the haematological indices of piglets. Matrine significantly reduced the abundance of CpA in faecal and intestines, as well as the content of toxin-α. The inflammatory response was mitigated following Matrine treatment, and physiological organ functions were restored, particularly through the repair of intestinal mucosal layer and sIgA level. Matrine also promoted a high abundance of beneficial intestinal microbiota, including Lactobacillus, Bacteroidota, Muribaculaceae, and Oscillospirales. This microbial composition inhibited the abundance of epithelial cells by Cp, thereby further reducing the diarrhoea rate and mortality in piglets. In conclusion, Matrine promotes the abundance of intestinal probiotics, inhibits the colonization of CpA, reduces the inflammatory response as well as restores intestinal tissue hismorphology. It will be used as a therapeutic agent diarrhea of piglets infected with CpA in the furture.
产气荚膜梭菌A型（Cp）是导致断奶仔猪细菌性腹泻的主要微生物之一。苦参碱是一种四环喹诺里西啶生物碱，具有多种生物活性，包括抗炎和神经保护作用。为了更好地了解苦参碱对感染A型产气荚膜梭菌（CpA）仔猪的抗炎机制，我们在14天的时间里研究了其对腹泻的预防作用。我们的结果表明，30mg/kg的苦参碱无毒，不会损害器官组织的组织结构，也不会改变仔猪的血液学指标。苦参碱显著降低了粪便和肠道中CpA的丰度以及毒素-α的含量。苦参碱治疗后炎症反应减轻，生理器官功能恢复，特别是通过修复肠黏膜层和sIgA水平。苦参碱还促进了包括乳酸杆菌、拟杆菌门、毛螺菌科和颤螺菌目的有益肠道微生物群的高丰度。这种微生物组成抑制了Cp对上皮细胞的丰度，从而进一步降低了仔猪的腹泻率和死亡率。总之，苦参碱促进肠道益生菌的丰度，抑制CpA的定植，减轻炎症反应并恢复肠道组织形态。它将在未来用作感染CpA的仔猪腹泻的治疗剂。
