Peng Yong, Guo Congshan, Yang Yanhong, Li Fenglin, Zhang Yanxia, Jiang Bin, Li Qingwang
Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, P.R. China.
Department of Oncology, The First Hospital of Qinhuangdao Town, Qinhuangdao, Hebei 066003, P.R. China.
Mol Med Rep. 2015 Mar;11(3):2129-34. doi: 10.3892/mmr.2014.2885. Epub 2014 Nov 6.
A number of studies have shown that baicalein shows high antitumor activity in vitro and in vivo. In this study, the inhibitory effect of baicalein on human cervical cancer HeLa cells was studied in vitro. HeLa cells were treated with high (100 µg/ml) and low (50 µg/ml) doses of baicalein, and cell growth inhibition rates were examined by the MTT assay. The morphological changes of apoptotic cells were observed under the light and electron microscope, while the rate of cell apoptosis was examined by flow cytometry. The expression of apoptosis-related proteins was analyzed by western blot, and caspase-3 activation was examined by a caspase-3 activity assay and spectrophotometry. The results demonstrated that baicalein inhibits the proliferation of HeLa cells and induces apoptosis in a caspase-3-dependent pathway, through downregulation of the B-cell lymphoma 2 (Bcl-2) protein and upregulation of the Bcl-2-associated X protein (Bax), Fas, Fas ligand (FasL) and caspase-8. Thus, we conclude that baicalein induces apoptosis of HeLa cells via the mitochondrial and the death receptor pathways. Cell apoptosis in HeLa cells was most likely promoted by the activation of the proteolytic enzyme caspase-3 in both pathways.
多项研究表明，黄芩苷在体外和体内均显示出较高的抗肿瘤活性。在本研究中，对黄芩苷在体外对人宫颈癌HeLa细胞的抑制作用进行了研究。用高剂量（100μg/ml）和低剂量（50μg/ml）的黄芩苷处理HeLa细胞，通过MTT法检测细胞生长抑制率。在光学显微镜和电子显微镜下观察凋亡细胞的形态变化，同时通过流式细胞术检测细胞凋亡率。通过蛋白质印迹法分析凋亡相关蛋白的表达，并通过caspase-3活性测定和分光光度法检测caspase-3的激活情况。结果表明，黄芩苷通过下调B细胞淋巴瘤2（Bcl-2）蛋白并上调Bcl-2相关X蛋白（Bax）、Fas、Fas配体（FasL）和caspase-8，抑制HeLa细胞的增殖并以caspase-3依赖性途径诱导细胞凋亡。因此，我们得出结论，黄芩苷通过线粒体途径和死亡受体途径诱导HeLa细胞凋亡。HeLa细胞中的细胞凋亡很可能是由两条途径中蛋白水解酶caspase-3的激活所促进的。
