用于大型植物群体遗传基因组学的多重数字基因表达分析

Multiplexed digital gene expression analysis for genetical genomics in large plant populations.

作者信息

Obermeier Christian, Salazar-Colqui Bertha M, Spamer Viola, Snowdon Rod

机构信息

Department of Plant Breeding, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, Giessen, 35392, Germany,

出版信息

Methods Mol Biol. 2015;1245:119-40. doi: 10.1007/978-1-4939-1966-6_9.

DOI:10.1007/978-1-4939-1966-6_9

PMID:25373753

Abstract

Digital gene expression (DGE) analysis is a cost-effective method for large-scale quantitative transcriptome analysis using second generation sequencing. Here we describe how adaptation of DGE with barcode indexing in large segregating plant populations of over 100 genotypes can be applied for successful expression QTL (eQTL) and gene expression network analysis to develop transcript-based markers for breeding.

摘要

数字基因表达（DGE）分析是一种利用第二代测序技术进行大规模定量转录组分析的经济高效的方法。在此，我们描述了如何将在超过100个基因型的大型分离植物群体中采用条形码索引的DGE方法应用于成功的表达数量性状基因座（eQTL）和基因表达网络分析，以开发基于转录本的育种标记。