He Ke, Hu Yun, Mao Xiaoming
Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Nov;30(11):1190-3.
To evaluate the changes of various immune regulatory cells and their subsets in peripheral blood mononuclear cells (PBMCs) of patients with Graves' disease (GD).
Peripheral blood cells were obtained from 32 GD patients and 30 normal subjects. The proportions of regulatory T cells (Tregs), T helper 1 (Th1) cells, Th2 cells, regulatory B cells (Bregs), dendritic cells (DCs) and their subsets in PBMCs were analyzed with flow cytometry.
Compared with the normal subjects, the proportions of Tregs, Bregs and total DCs significantly decreased in PBMCs of the GD patients. However, the proportions of Th1, Th2 cells in PBMCs significantly increased in the GD patients compared with the normal subjects. As for DC subsets, the proportion of myeloid DCs (mDCs) dropped and plasmacytoid DCs (pDCs) went up in PBMCs of the GD patients compared with the normal subjects. Moreover, the proportion of mature DCs was raised in total DCs in the GD patients compared with normal subjects.
There are disorders in various immune regulatory cells in PBMCs of the GD patients, which may play important roles in the onset and progression of GD.
评估格雷夫斯病（GD）患者外周血单个核细胞（PBMC）中各种免疫调节细胞及其亚群的变化。
采集32例GD患者和30例正常受试者的外周血细胞。采用流式细胞术分析PBMC中调节性T细胞（Tregs）、辅助性T细胞1（Th1）、辅助性T细胞2（Th2）、调节性B细胞（Bregs）、树突状细胞（DCs）及其亚群的比例。
与正常受试者相比，GD患者PBMC中Tregs、Bregs和总DCs的比例显著降低。然而，与正常受试者相比，GD患者PBMC中Th1、Th2细胞的比例显著升高。至于DC亚群，与正常受试者相比，GD患者PBMC中髓样DC（mDCs）的比例下降，浆细胞样DC（pDCs）的比例上升。此外，与正常受试者相比，GD患者总DCs中成熟DCs的比例升高。
GD患者PBMC中各种免疫调节细胞存在紊乱，这可能在GD的发病和进展中起重要作用。
