Monos D S, Frank T S, Senior M B, Gadson M, Zmijewski C M, Prystowsky M B, Goodman D B
Department of Pathology, University of Pennsylvania Medical School, Philadelphia.
Transfusion. 1989 Feb;29(2):119-23. doi: 10.1046/j.1537-2995.1989.29289146828.x.
Four patients with a history of multiple blood transfusions who awaited renal transplantation were tested for human immunodeficiency virus (HIV) infection and found to be positive on enzyme immunoassay (EIA) and negative on Western blot. None of these patients had any clinical evidence of HIV infection. Absorption of these patients' sera with B-lymphoblastoid cell lines (B-LCL) positive for the serologic specificities DR3, DR4 (Dw4, Dw10, Dw14), and DR5 resulted in EIAs that were negative for HIV. Treatment of the B-LCL with an anti-DR monoclonal antibody (L243) interfered with the absorption of the serum sample by B-LCL. This indicates that the initial false-positive EIA results may be due to HLA antibodies. Furthermore, it was shown that these HLA antibodies are not limited in specificity to the HLA type of the host cell used in the preparation of the EIA reagents, but can consist of other DR specificities.
对4例等待肾移植且有多次输血史的患者进行了人类免疫缺陷病毒（HIV）感染检测，酶免疫测定（EIA）结果呈阳性，而蛋白质印迹法结果为阴性。这些患者均无HIV感染的任何临床证据。用血清学特异性为DR3、DR4（Dw4、Dw10、Dw14）和DR5阳性的B淋巴母细胞系（B-LCL）吸收这些患者的血清后，EIA检测HIV结果为阴性。用抗DR单克隆抗体（L243）处理B-LCL会干扰B-LCL对血清样本的吸收。这表明最初的EIA假阳性结果可能是由于HLA抗体所致。此外，研究表明这些HLA抗体的特异性并不局限于用于制备EIA试剂的宿主细胞的HLA类型，还可能包括其他DR特异性。
