血流感染气单胞菌分离株的毒力多样性：体外、动物及临床证据
Virulence diversity among bacteremic Aeromonas isolates: ex vivo, animal, and clinical evidences.
作者信息
Chen Po-Lin, Wu Chi-Jung, Tsai Pei-Jane, Tang Hung-Jen, Chuang Yin-Ching, Lee Nan-Yao, Lee Ching-Chi, Li Chia-Wen, Li Ming-Chi, Chen Chi-Chung, Tsai Hung-Wen, Ou Chun-Chun, Chen Chang-Shi, Ko Wen-Chien
机构信息
Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan; Graduate Institute of Clinical Medicine, National Cheng Kung University College of Medicine, Tainan, Taiwan.
Graduate Institute of Clinical Medicine, National Cheng Kung University College of Medicine, Tainan, Taiwan; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Taipei, Taiwan.
出版信息
PLoS One. 2014 Nov 6;9(11):e111213. doi: 10.1371/journal.pone.0111213. eCollection 2014.
BACKGROUND
The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.
METHODOLOGY/PRINCIPAL FINDINGS: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5% vs. 0%, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values <0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values <0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values <0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.
CONCLUSIONS/SIGNIFICANCE: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.
背景
本研究的目的是比较引起血流感染的不同气单胞菌属菌种的毒力。
方法/主要发现：从四种气单胞菌血液分离株中随机选取九株进行分析，包括达卡气单胞菌、嗜水气单胞菌、维罗纳气单胞菌和豚鼠气单胞菌。通过rpoD的DNA序列匹配来鉴定菌种。临床上，达卡气单胞菌菌血症患者的脓毒症相关死亡率高于其他菌种患者（37.5%对0%，P = 0.028）。在秀丽隐杆线虫、小鼠成纤维细胞C2C12细胞系和BALB/c小鼠模型中测试了不同气单胞菌属菌种的毒力。与其他菌种相比，喂食达卡气单胞菌和豚鼠气单胞菌的秀丽隐杆线虫的存活率分别最低和最高（所有P值<0.0001）。达卡气单胞菌分离株在C2C12细胞系中也表现出更强的细胞毒性（所有P值<0.0001）。肌肉注射达卡气单胞菌的小鼠的14天存活率低于其他菌种（所有P值<0.0001）。豚鼠气单胞菌分离株中很少检测到溶血活性和几个毒力因子基因。
结论/意义：临床数据、体外实验和动物研究表明，临床上重要的气单胞菌属菌种之间存在毒力差异。
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