Roges Emily Moraes, Gonçalves Veronica Dias, Rodrigues Marcelle da Silva, Festivo Marcia Lima, Ott Paulo Henrique, Araujo André Luiz, Siciliano Salvatore, Berto Lucia Helena, Aquino Maria Helena Cosendey de, Rodrigues Dalia Dos Prazeres
Laboratório de Referência Nacional em Enteroinfecções Bacterianas/Laboratório de Enterobactérias, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040361, RJ, Brazil.
Programa de Pós-graduação em Higiene Veterinária e Processamento de Produtos de Origem Animal, Faculdade de Medicina Veterinária, Universidade Federal Fluminense (UFF), Niterói 24230340, RJ, Brazil.
Microorganisms. 2025 Aug 8;13(8):1851. doi: 10.3390/microorganisms13081851.
and are ubiquitous microorganisms, widespread in aquatic environments, and can cause severe infections in humans and animals. This study aimed to determine the diversity of virulence genes A, A, , and through polymerase chain reaction and the antimicrobial resistance through disk diffusion test of 101 and 34 strains from environmental, animal, and human sources gathered between 2016 and 2019 at the National Reference Laboratory for Enteric Diseases. Overall, the virulence gene distribution was in 35.5% of the samples, in 40.7%, A in 42.2%, and A in 44.5%. Our results revealed that 76.3% of the 135 isolated exhibited at least one of the genes above. 76.3% of and 76.5% of exhibited virulence genes distributed among 15 and 12 virulence profiles, respectively. Antimicrobial resistance was observed in 86% of the strains (87.1% in and 82.4% in ), with higher rates of resistance to Nalidixic acid (69.3%), Imipenem (31.1%), and Sulfamethoxazole-trimethoprim (15.5%). The occurrence of virulence genes and antimicrobial resistance in and from different sources indicates their diversity and pathogenicity, reinforcing that they can be a potential health risk source.
[具体微生物名称1]和[具体微生物名称2]是普遍存在的微生物，广泛分布于水生环境中，可导致人类和动物发生严重感染。本研究旨在通过聚合酶链反应确定[具体微生物名称1]和[具体微生物名称2]毒力基因A、A、[具体毒力基因3]和[具体毒力基因4]的多样性，并通过纸片扩散法检测2016年至2019年期间在国家肠道疾病参考实验室收集的来自环境、动物和人类来源的101株[具体微生物名称1]和34株[具体微生物名称2]的抗菌药物耐药性。总体而言，毒力基因分布情况为：[具体毒力基因1]在35.5%的样本中存在，[具体毒力基因2]在40.7%的样本中存在，A在42.2%的样本中存在，A在44.5%的样本中存在。我们的结果显示，135株分离出的[具体微生物名称1]和[具体微生物名称2]中，76.3%至少含有上述一种基因。76.3%的[具体微生物名称1]和76.5%的[具体微生物名称2]表现出分别分布于15种和12种毒力谱中的毒力基因。在86%的菌株中观察到抗菌药物耐药性（[具体微生物名称1]中为87.1%，[具体微生物名称2]中为82.4%），对萘啶酸（69.3%）、亚胺培南（31.1%）和磺胺甲恶唑-甲氧苄啶（15.5%）的耐药率较高。来自不同来源的[具体微生物名称1]和[具体微生物名称2]中毒力基因和抗菌药物耐药性的出现表明它们具有多样性和致病性，进一步证明它们可能是潜在的健康风险源。
