Nunes Pauli Gisele Elias, de la Escosura-Muñiz Alfredo, Parolo Claudio, Helmuth Bechtold Ivan, Merkoçi Arben
ICN2 - Nanobioelectronics & Biosensors Group, Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Spain.
Lab Chip. 2015 Jan 21;15(2):399-405. doi: 10.1039/c4lc01123f.
We have developed a paper and gold nanoparticle (AuNP)-based lab-in-a-syringe (LIS) for immunosensing of biomarkers. This simple diagnostic device features simultaneous sampling and vertical-flow operation, which means that unlike typical immunosensors, it does not suffer from any delay between sampling and detection. It can handle large-volume, low-concentration samples for analysis in diverse applications (e.g. biomedical, environmental, food, etc.). Furthermore, its operating range for sample concentration can be tuned by simply changing the volume of the syringed sample, which enables on-demand limits of detection (LOD). The LIS contains two nitrocellulose pads: the conjugate pad (which captures the analyte) and the detection pad (which signals the presence of the captured analyte) both embedded into reusable plastic cartridges. We demonstrated its efficiency in detecting human IgG (HIgG) (LOD: 1.0 ng mL(-1)) and prostate-specific antigen (PSA) (spiked urine samples; LOD: 1.9 ng mL(-1)). In the field, the LIS can be used for complete on-site analysis or to obtain partially analyzed samples (AuNPs with captured analyte) for subsequent detailed testing in specialized laboratories.
我们开发了一种基于纸张和金纳米颗粒（AuNP）的注射器式实验室（LIS），用于生物标志物的免疫传感。这种简单的诊断设备具有同步采样和垂直流动操作的特点，这意味着与典型的免疫传感器不同，它在采样和检测之间不存在任何延迟。它可以处理大量低浓度样本，用于各种应用（如生物医学、环境、食品等）的分析。此外，通过简单地改变注射器中样本的体积，可以调整其样本浓度的工作范围，从而实现按需检测限（LOD）。该LIS包含两个硝酸纤维素垫：结合垫（捕获分析物）和检测垫（指示捕获的分析物的存在），两者都嵌入可重复使用的塑料盒中。我们证明了其在检测人IgG（HIgG）（LOD：1.0 ng mL(-1)）和前列腺特异性抗原（PSA）（加标尿液样本；LOD：1.9 ng mL(-1)）方面的效率。在现场，LIS可用于完整的现场分析，或获取部分分析的样本（带有捕获分析物的AuNP），以便在专业实验室进行后续的详细测试。
