Narware Jeetu, Chakma Jharjhari, Singh Satyendra P, Prasad Divya Raj, Meher Jhumishree, Singh Prachi, Bhargava Priya, Sawant Shraddha Bhaskar, Singh Jyoti P, Manzar Nazia, Kashyap Abhijeet Shankar
Department of Mycology and Plant Pathology, Institute of Agriculture Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Department of Kayachikitsa, Faculty Ayurveda, Institute of Medical sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Front Bioeng Biotechnol. 2025 Apr 23;13:1570318. doi: 10.3389/fbioe.2025.1570318. eCollection 2025.
Nanotechnology has significantly advanced the detection of plant diseases by introducing nano-inspired biosensors that offer distinct advantages over traditional diagnostic methods. These biosensors, enhanced with novel nanomaterials, exhibit increased sensitivity, catalytic activity, and faster response times, resulting in improved diagnostic efficiency. The increasing impact of climate-induced stress and emerging plant pathogens have created an urgent demand for real-time monitoring systems in agriculture. Nanobiosensors are revolutionizing plant disease management by enabling on-site detection of pests and weeds, facilitating precise pesticide applications. This article provides a comprehensive overview of the development and application of nanobiosensors in real-time plant disease diagnosis. It highlights key innovations, such as smartphone-integrated nanozyme biosensing and lab-on-a-chip technologies. Special emphasis is placed on the detection of molecular biomarkers, demonstrating the critical role of nanobiosensors in addressing the evolving challenges of plant disease management and agricultural sustainability.
纳米技术通过引入受纳米启发的生物传感器，显著推动了植物病害检测的发展，这些生物传感器相较于传统诊断方法具有明显优势。这些用新型纳米材料增强的生物传感器，具有更高的灵敏度、催化活性和更快的响应时间，从而提高了诊断效率。气候诱导的压力和新出现的植物病原体的影响日益增大，对农业实时监测系统产生了迫切需求。纳米生物传感器通过实现对害虫和杂草的现场检测，推动精确施用农药，正在彻底改变植物病害管理。本文全面概述了纳米生物传感器在实时植物病害诊断中的发展与应用。它突出了关键创新，如集成智能手机的纳米酶生物传感和芯片实验室技术。特别强调了分子生物标志物的检测，证明了纳米生物传感器在应对植物病害管理和农业可持续发展不断演变的挑战中的关键作用。
