Schmid C, Zapf J, Froesch E R
Metabolic Unit, CL, University Hospital, Zurich, Switzerland.
FEBS Lett. 1989 Feb 27;244(2):328-32. doi: 10.1016/0014-5793(89)80556-3.
A bone-derived rat cell line, PyMS, releases IGF I and IGF carrier proteins which are similar to those found in rat serum. Western blot analysis of culture media conditioned by hormone-treated cells shows that growth hormone and IGF I stimulate and cortisol inhibits production of IGF carrier proteins in vitro. A glycosylated carrier protein species of 49-42 kDa is closely related to the subunits of the growth hormone-dependent carrier protein complex found in rat serum. In addition, rhIGF I rapidly induces a 32 kDa, non-glycosylated IGF-binding protein whose accumulation is markedly increased by cortisol.
一种源自大鼠骨骼的细胞系PyMS可释放胰岛素样生长因子I（IGF I）和IGF载体蛋白，这些蛋白与大鼠血清中的蛋白相似。对经激素处理的细胞所分泌的培养基进行蛋白质免疫印迹分析表明，生长激素和IGF I在体外具有刺激作用，而皮质醇则具有抑制作用，它们能够调节IGF载体蛋白的产生。一种49 - 42 kDa的糖基化载体蛋白与大鼠血清中依赖生长激素的载体蛋白复合物的亚基密切相关。此外，重组人IGF I（rhIGF I）能快速诱导一种32 kDa的非糖基化IGF结合蛋白的产生，皮质醇可显著增加该蛋白的积累。
