Teo C G, Wong S Y, Best P V
Department of Virology, Royal Postgraduate Medical School, London, U.K.
J Pathol. 1989 Feb;157(2):135-40. doi: 10.1002/path.1711570208.
JC virus genomes have been localized in formalin-fixed, paraffin-embedded brain tissues of two cases of known progressive multifocal leukoencephalopathy by in situ hybridization utilizing a biotinylated JC virus DNA probe. A three-stage immunoperoxidase system with gold-silver amplification of the diaminobenzidine substrate was used to visualize biotinylated nucleic acid hybrids. Dot-blot quantification of this visualization system indicates that subpicogramme amounts of biotinylated DNA can be detected. Optimal detection of the virus genomes in the brain tissues required a microwave irradiation step prior to hybridization. JC virus genomes were observed in the nuclei of enlarged oligodendrocytes and of some bizarre astrocytes. No other cell types were found to harbour the genomes.
通过使用生物素化的JC病毒DNA探针进行原位杂交，在两例已知的进行性多灶性白质脑病的福尔马林固定、石蜡包埋脑组织中定位了JC病毒基因组。使用具有二氨基联苯胺底物金银放大的三步免疫过氧化物酶系统来可视化生物素化的核酸杂交体。该可视化系统的斑点印迹定量表明，可以检测到亚皮克级量的生物素化DNA。在杂交之前，脑组织中病毒基因组的最佳检测需要微波照射步骤。在增大的少突胶质细胞和一些怪异星形胶质细胞的细胞核中观察到JC病毒基因组。未发现其他细胞类型含有该基因组。
