Aksamit A J, Mourrain P, Sever J L, Major E O
Ann Neurol. 1985 Oct;18(4):490-6. doi: 10.1002/ana.410180412.
Using the technique of in situ DNA-to-DNA hybridization, a JC virus biotinylated DNA probe was developed and applied to formalin-fixed, paraffin-embedded, or fixed, frozen sections of brain tissue from three subjects with progressive multifocal leukoencephalopathy (PML). Light microscopy was carried out to correlate the presence of JC virus DNA with the selective infection of oligodendrocytes and astrocytes in PML. Oligodendrocytes (lytically infected) showed the greatest evidence of viral DNA. More astrocytes showing bizarre morphological changes had evidence of viral DNA than did astrocytes that were simply reactive. Viral DNA was not evident in vascular endothelial cells using this technique. Viral DNA replication may be an important initial step which produces the bizarre "transformed" astrocytes of PML. Findings in this study do not support the hypothesis that vascular endothelial replication is important in the pathogenesis of JC virus-induced PML. In situ hybridization with biotinylated JC virus probe may be useful in the diagnosis of PML on brain biopsy specimens.
运用原位DNA与DNA杂交技术，研制出一种JC病毒生物素化DNA探针，并将其应用于来自三名进行性多灶性白质脑病（PML）患者的福尔马林固定、石蜡包埋或固定冷冻的脑组织切片。进行光学显微镜检查，以关联JC病毒DNA的存在与PML中少突胶质细胞和星形胶质细胞的选择性感染。少突胶质细胞（溶解性感染）显示出病毒DNA的最明显证据。与单纯反应性星形胶质细胞相比，更多呈现奇异形态变化的星形胶质细胞有病毒DNA的证据。使用该技术在血管内皮细胞中未发现病毒DNA。病毒DNA复制可能是产生PML中奇异“转化”星形胶质细胞的重要初始步骤。本研究结果不支持血管内皮复制在JC病毒诱导的PML发病机制中起重要作用的假说。用生物素化JC病毒探针进行原位杂交可能有助于脑活检标本中PML的诊断。
