Ueki K, Richardson E P, Henson J W, Louis D N
Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Boston.
Ann Neurol. 1994 Oct;36(4):670-3. doi: 10.1002/ana.410360421.
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease that is caused by JC papovavirus. The virus can be demonstrated in brains with PML using a variety of techniques. In situ polymerase chain reaction (PCR) is a new method that combines the sensitivity of PCR with the histological localization of in situ hybridization. We here show that in situ PCR can detect JC virus in archival tissue sections of 3 cases of PML, including 1 of the original cases described in 1958 and 1 case that was fixed in formalin for 7 weeks. JC virus DNA was amplified directly on tissue and demonstrated in the characteristic enlarged oligodendrocytes and bizarre astrocytes. These data illustrate the utility of the in situ PCR technique in detecting JC virus in archival sections, including those fixed for prolonged periods, and provide a historical footnote to the original report of PML.
进行性多灶性白质脑病（PML）是一种由JC多瘤病毒引起的脱髓鞘疾病。使用多种技术可在患有PML的大脑中检测到该病毒。原位聚合酶链反应（PCR）是一种将PCR的敏感性与原位杂交的组织学定位相结合的新方法。我们在此表明，原位PCR可在3例PML的存档组织切片中检测到JC病毒，其中包括1958年描述的首例病例中的1例以及1例用福尔马林固定7周的病例。JC病毒DNA在组织上直接扩增，并在特征性增大的少突胶质细胞和怪异的星形胶质细胞中得以显示。这些数据说明了原位PCR技术在检测存档切片（包括长时间固定的切片）中的JC病毒方面的实用性，并为PML的原始报告提供了一个历史注释。
