两种具有不同扩散模式的附生地衣中的微卫星位点：Nephroma laevigatum 和 N. parile（Nephromataceae）。
Microsatellite loci in two epiphytic lichens with contrasting dispersal modes: Nephroma laevigatum and N. parile (Nephromataceae).
机构信息
Royal Botanic Garden Edinburgh, 20A Inverleith Row, Scotland, United Kingdom.
出版信息
Appl Plant Sci. 2014 Nov 3;2(11). doi: 10.3732/apps.1400080. eCollection 2014 Nov.
PREMISE OF THE STUDY
Microsatellite markers were characterized for two epiphytic cyanolichens, Nephroma laevigatum and N. parile (Nephromataceae), and will be used to investigate population structure and estimate gene flow among populations of these two closely related species with contrasting dispersal modes. •
METHODS AND RESULTS
Twelve and 14 microsatellite loci were characterized for N. laevigatum and N. parile, respectively. Allele number in N. laevigatum ranged from three to 13 per locus, while in N. parile there were from two to six alleles per locus. As expected, the sexually reproducing N. laevigatum had higher genetic diversity than the predominantly asexual N. parile. •
CONCLUSIONS
This new set of markers is suitable for studying population structure and providing insights into gene flow among populations and for understanding processes of diversification. Compared between the species, they will facilitate an understanding of the influence of contrasting reproductive strategies on population and community structure.
研究前提
为两种附生蓝藻，Nephroma laevigatum 和 N. parile（Nephromataceae），表征了微卫星标记，并将用于研究这些具有不同扩散模式的两个密切相关物种的种群结构和估计种群间基因流。
方法和结果
分别为 N. laevigatum 和 N. parile 表征了 12 个和 14 个微卫星位点。N. laevigatum 每个位点的等位基因数范围为 3 到 13，而 N. parile 每个位点的等位基因数为 2 到 6。正如预期的那样，有性繁殖的 N. laevigatum 的遗传多样性高于主要无性繁殖的 N. parile。
结论
这套新的标记物适合用于研究种群结构，洞察种群间的基因流，并理解多样化过程。与物种相比，它们将有助于理解不同繁殖策略对种群和群落结构的影响。
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