Chung Jin-Yong, Lim Hyoun-Ju, Kim Young-Jin, Song Ki-Hoon, Kim Byoung-Gwon, Hong Young-Seoub
Heavy Metal Exposure Environmental Health Center, Dong-A University, Busan, Korea.
Agilent Technologies Korea Ltd., Seoul, Korea.
Environ Health Toxicol. 2014 Oct 30;29:e2014018. doi: 10.5620/eht.e2014018. eCollection 2014.
The purpose of this study was to determine a separation method for each arsenic metabolite in urine by using a high performance liquid chromatography (HPLC)- inductively coupled plasma-mass spectrometer (ICP-MS).
Separation of the arsenic metabolites was conducted in urine by using a polymeric anion-exchange (Hamilton PRP X-100, 4.6 mm×150 mm, 5 μm) column on Agilent Technologies 1260 Infinity LC system coupled to Agilent Technologies 7700 series ICP/MS equipment using argon as the plasma gas.
All five important arsenic metabolites in urine were separated within 16 minutes in the order of arsenobetaine, arsenite, dimethylarsinate, monomethylarsonate and arsenate with detection limits ranging from 0.15 to 0.27 μg/L (40 μL injection). We used GEQUAS No. 52, the German external quality assessment scheme and standard reference material 2669, National Institute of Standard and Technology, to validate our analyses.
The method for separation of arsenic metabolites in urine was established by using HPLC-ICP-MS. This method contributes to the evaluation of arsenic exposure, health effect assessment and other bio-monitoring studies for arsenic exposure in South Korea.
本研究的目的是使用高效液相色谱（HPLC）-电感耦合等离子体质谱仪（ICP-MS）确定尿液中每种砷代谢物的分离方法。
采用聚合物阴离子交换柱（Hamilton PRP X-100，4.6 mm×150 mm，5μm）在安捷伦科技1260 Infinity LC系统上对尿液中的砷代谢物进行分离，并与安捷伦科技7700系列ICP/MS设备联用，以氩气作为等离子体气体。
尿液中所有五种重要的砷代谢物在16分钟内按砷甜菜碱、亚砷酸盐、二甲基砷酸盐、一甲基砷酸盐和砷酸盐的顺序分离，检测限为0.15至0.27μg/L（进样量40μL）。我们使用德国外部质量评估计划GEQUAS No. 52和美国国家标准与技术研究院的标准参考物质2669对我们的分析进行验证。
采用HPLC-ICP-MS建立了尿液中砷代谢物的分离方法。该方法有助于韩国对砷暴露的评估、健康效应评估以及其他砷暴露生物监测研究。
