National Institute of Standards and Technology (NIST), Analytical Chemistry Division, Hollings Marine Laboratory, 331 Fort Johnson Road, Charleston, SC 29412, USA.
Anal Bioanal Chem. 2010 Apr;396(8):3041-50. doi: 10.1007/s00216-010-3541-y. Epub 2010 Feb 26.
Two independent liquid chromatography inductively coupled plasma-mass spectrometry (LC/ICP-MS) methods for the separation of arsenic species in urine have been developed with quantification by standard additions. Seven arsenic species have been quantified in a new NIST frozen human urine Standard Reference Material (SRM) 2669 Arsenic Species in Frozen Human Urine, Levels 1 and 2. The species measured were: arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), dimethylarsinate (DMA), arsenobetaine (AB), arsenocholine (AC), and trimethylarsine oxide (TMAO). The purity of each arsenic standard used for quantification was measured as well as the arsenic species impurities determined in each standard. Analytical method limits of detection (L(D)) for the various species in both methods ranged from 0.2 to 0.8 microg L(-1) as arsenic. The results demonstrate that LC/ICP-MS is a sensitive, reproducible, and accurate technique for the determination of low-level arsenic species in urine. Measurements of the arsenic species 3 years after initial production of the SRM demonstrate the stability of the arsenic species in the urine reference material.
已开发出两种独立的液相色谱-电感耦合等离子体质谱（LC/ICP-MS）方法，可用于分离尿液中的砷形态，并通过标准添加进行定量。在新的 NIST 冷冻人尿标准参考物质（SRM）2669 中，已定量测量了七种砷形态，包括冷冻人尿中的砷形态，水平 1 和 2。测量的形态包括：亚砷酸盐（As（III））、砷酸盐（As（V））、一甲基砷酸（MMA）、二甲基砷酸（DMA）、砷甜菜碱（AB）、砷胆碱（AC）和三甲基胂氧化物（TMAO）。还测量了每种用于定量的砷标准的纯度，以及每种标准中确定的砷形态杂质。两种方法中各种形态的分析方法检测限（L（D））均为 0.2 至 0.8 μg L（-1）作为砷。结果表明，LC/ICP-MS 是一种灵敏、重现性好且准确的技术，可用于测定尿液中的低水平砷形态。在 SRM 最初生产三年后对砷形态的测量表明，尿液参考物质中砷形态的稳定性。
