Pohl J, Saltsman I, Mahammed A, Gross Z, Röder B
Department of Physics, Humboldt - Universität zu Berlin, Berlin, Germany.
J Appl Microbiol. 2015 Feb;118(2):305-12. doi: 10.1111/jam.12690. Epub 2014 Dec 11.
This study was performed to examine the potential of photodynamic inactivation for growth inhibition of green algae through generation of singlet oxygen.
Two cationic and two anionic corroles were investigated according to their photoinhibitive effect on two strains of green algae using visible light for photoexcitation. The development of biomass over the experimental period of 18 days was followed using absorptive properties of the algae samples. The anionic photosensitizers showed no significant phototoxicity, whereas the cationic photosensitizers caused a drastic reduction of biomass on a short time scale and also displayed long-term inhibition of algae growth.
In general, it was proven that photodynamic inactivation of green algae is possible. Concluding from the results of this study, cationic photosensitizers are favourable for this task, while anionic photosensitizers are not suited.
Phototrophic biofilms are an important factor in biofouling and biodeterioration of building materials, causing great damage to historic and contemporary constructions. Growth inhibition of phototrophic organisms using photodynamic inactivation could pose an alternative to the use of biocides. To this end, successful application of this approach on green algae is a vital step in the development of suitable photosensitizers.
本研究旨在通过单线态氧的产生来检验光动力失活对绿藻生长抑制的潜力。
根据两种阳离子和两种阴离子卟吩对两株绿藻的光抑制作用，利用可见光进行光激发来研究它们。在18天的实验期内，利用藻类样品的吸收特性跟踪生物量的变化。阴离子光敏剂未显示出明显的光毒性，而阳离子光敏剂在短时间内导致生物量急剧减少，并且还表现出对藻类生长的长期抑制作用。
总体而言，已证明绿藻的光动力失活是可行的。从本研究结果得出结论，阳离子光敏剂有利于此项任务，而阴离子光敏剂则不适用。
光合生物膜是建筑材料生物污损和生物劣化的一个重要因素，对历史建筑和当代建筑造成巨大破坏。利用光动力失活抑制光合生物的生长可能是使用杀菌剂的一种替代方法。为此，该方法在绿藻上的成功应用是开发合适光敏剂的关键一步。
