超氧阴离子自由基在血液单核细胞衍生的人巨噬细胞乙醇代谢中的作用。
Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages.
作者信息
Wickramasinghe S N
机构信息
Department of Haematology, St. Mary's Hospital Medical School, London, United Kingdom.
出版信息
J Exp Med. 1989 Mar 1;169(3):755-63. doi: 10.1084/jem.169.3.755.
The effects of a number of additives on the rate of conversion of ethanol (1 mg/ml; 21.7 mM) to acetate by monolayers of blood monocyte-derived human macrophages were investigated. The additives studied were superoxide dismutase (SOD; 1,500 U/ml), catalase (1,500 U/ml), tetrahydrofurane (20 mM), and PMA (20 nM), either singly or in various combinations. SOD, catalase, SOD plus catalase, tetrahydrofurane, and tetrahydrofurane plus SOD inhibited ethanol oxidation by 49.2, 12.1, 52.9, 60.4, and 66.8%, respectively. PMA caused a 4.0-8.3-fold increase in the rate of ethanol metabolism and this increase was completely suppressed in the presence of SOD. The data indicate that a substantial proportion of the ethanol metabolism by both unstimulated and PMA-stimulated blood monocyte-derived macrophages was dependent on the generation of superoxide anion radicals.
研究了多种添加剂对人血单核细胞衍生的巨噬细胞单层将乙醇（1毫克/毫升；21.7毫摩尔）转化为乙酸盐速率的影响。所研究的添加剂为超氧化物歧化酶（SOD；1500单位/毫升）、过氧化氢酶（1500单位/毫升）、四氢呋喃（20毫摩尔）和佛波酯（20纳摩尔），单独使用或各种组合使用。SOD、过氧化氢酶、SOD加过氧化氢酶、四氢呋喃以及四氢呋喃加SOD分别使乙醇氧化受到49.2%、12.1%、52.9%、60.4%和66.8%的抑制。佛波酯使乙醇代谢速率提高了4.0至8.3倍，而在SOD存在的情况下这种提高被完全抑制。数据表明，未刺激和佛波酯刺激的血单核细胞衍生巨噬细胞对乙醇的代谢很大程度上依赖于超氧阴离子自由基的产生。
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