Bond A N, Wickramasinghe S N
Acta Haematol. 1983;69(5):303-13. doi: 10.1159/000206911.
The metabolism of ethanol to acetate and CO2 by suspension cultures of human blood and marrow cells has been investigated. The average rate of metabolism of ethanol by relatively pure preparations of monocytes plus lymphocytes, neutrophils and erythrocytes obtained from normal peripheral blood were, respectively, 12.58, 5.44 and 0.32 nmol/10(7) cells/h, when the concentration of ethanol in the culture was 2.63 mM. Under similar culture conditions, the average rate of metabolism of ethanol by suspension cultures of human marrow cells was 19.0 nmol/10(7) nucleated marrow cells/h. The metabolism of ethanol by nucleated marrow cells was only slightly inhibited by pyrazole and 3-amino-1,2,4-triazole indicating that it was largely independent of pyrazole-sensitive alcohol dehydrogenase and catalase. By contrast, the oxidation of ethanol by isolated rat hepatocytes was markedly inhibited by pyrazole and therefore appeared to be mainly dependent on alcohol dehydrogenase. It is concluded that bone marrow cells have a considerable capacity to metabolize ethanol and that the predominant biochemical pathway involved in this metabolism is different from that involved in rat hepatocytes.
已对人血细胞和骨髓细胞悬浮培养物将乙醇代谢为乙酸盐和二氧化碳的过程进行了研究。当培养物中乙醇浓度为2.63 mM时，从正常外周血获得的相对纯的单核细胞加淋巴细胞、中性粒细胞和红细胞制剂对乙醇的平均代谢速率分别为12.58、5.44和0.32 nmol/10⁷细胞/小时。在相似的培养条件下，人骨髓细胞悬浮培养物对乙醇的平均代谢速率为19.0 nmol/10⁷有核骨髓细胞/小时。有核骨髓细胞对乙醇的代谢仅受到吡唑和3-氨基-1,2,4-三唑的轻微抑制，这表明该代谢在很大程度上不依赖于对吡唑敏感的乙醇脱氢酶和过氧化氢酶。相比之下，分离的大鼠肝细胞对乙醇的氧化受到吡唑的显著抑制，因此似乎主要依赖于乙醇脱氢酶。得出的结论是，骨髓细胞具有相当大的乙醇代谢能力，并且参与这种代谢的主要生化途径与大鼠肝细胞中的不同。
