Wickramasinghe S N, Barden G, Levy L
Alcohol Alcohol. 1987;22(1):31-9.
Tissue macrophages obtained from liver, bone marrow, spleen and thymus of C57 BL/6 mice closely resembled blood-monocyte-derived human macrophages in three characteristics. These were: the rate of metabolism of ethanol to acetate, the biochemical pathways involved in ethanol metabolism and the ability to generate an ethanol-dependent non-dialysable cytotoxic activity in vitro. The metabolism of ethanol by all four types of murine tissue macrophage was only slightly suppressed by pyrazole, 4-iodopyrazole and 3-amino-1,2,4-triazole, which are known to inhibit alcohol dehydrogenase (ADH), pi ADH and catalase respectively. By contrast, ethanol metabolism by these cells was strongly suppressed by three inhibitors of the cytochrome P-450-dependent microsomal ethanol-oxidising system--namely, carbon monoxide, metyrapone and tetrahydrofurane.
从C57 BL/6小鼠的肝脏、骨髓、脾脏和胸腺中获取的组织巨噬细胞在三个特征上与血液单核细胞衍生的人类巨噬细胞极为相似。这三个特征分别是：乙醇代谢为乙酸盐的速率、乙醇代谢所涉及的生化途径以及在体外产生乙醇依赖性非透析性细胞毒性活性的能力。所有四种类型的小鼠组织巨噬细胞对乙醇的代谢仅受到吡唑、4-碘吡唑和3-氨基-1,2,4-三唑的轻微抑制，已知这三种物质分别抑制乙醇脱氢酶（ADH）、πADH和过氧化氢酶。相比之下，这些细胞的乙醇代谢受到细胞色素P-450依赖性微粒体乙醇氧化系统的三种抑制剂——即一氧化碳、甲吡酮和四氢呋喃的强烈抑制。
