Hwang C Y, Lee I, Cho Y, Lee Y M, Baek K, Jung Y-J, Yang Y Y, Lee T, Rhee T S, Lee H K
Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
Department of Environmental Science, Hankuk University of Foreign Studies, 81 Oedae-ro, Yongin-si, Gyeonggi-do 449-791, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):465-471. doi: 10.1099/ijs.0.070086-0. Epub 2014 Nov 10.
A Gram-stain-positive, rod-shaped and non-motile strain, designated PAMC 27367(T), was isolated from rainwater collected on the Bering Sea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Rhodococcus. Phylogenetic analyses revealed that strain PAMC 27367(T) formed a robust clade with the type strains of Rhodococcus rhodnii, Rhodococcus aetherivorans and Rhodococcus ruber with 16S rRNA gene sequence similarities of 96.3 %, 95.8 % and 95.5 %, respectively. Cells of the strain grew optimally at 25 °C and at pH 6.5-7.0 in the presence of 0-2 % (w/v) sea salts. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and three unknown phospholipids. The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω8c and 10-methyl C17 : 0. Cell wall analysis showed that strain PAMC 27367(T) contained meso-diaminopimelic acid. The genomic DNA G+C content was 77.1 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Rhodococcus aerolatus sp. nov., with PAMC 27367(T) ( = KCTC 29240(T) = JCM 19485(T)) as the type strain.
从白令海采集的雨水中分离出一株革兰氏染色阳性、杆状且无运动性的菌株，命名为PAMC 27367(T)。对该菌株的16S rRNA基因序列分析表明它与红球菌属相关。系统发育分析显示，菌株PAMC 27367(T)与红褐红球菌、食醚红球菌和红平红球菌的模式菌株形成一个稳健的进化分支，其16S rRNA基因序列相似性分别为96.3%、95.8%和95.5%。该菌株细胞在25℃、pH 6.5 - 7.0且含有0 - 2%（w/v）海盐的条件下生长最佳。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和三种未知磷脂。主要细胞脂肪酸（＞10%）为异C16 : 0、C17 : 1ω8c和10 - 甲基C17 : 0。细胞壁分析表明菌株PAMC 27367(T)含有内消旋二氨基庚二酸。基因组DNA的G + C含量为77.1 mol%。基于此处呈现的系统发育、化学分类和表型数据，我们提出一个新物种，命名为气生红球菌（Rhodococcus aerolatus sp. nov.），以PAMC 27367(T)（= KCTC 29240(T)= JCM 19485(T)）作为模式菌株。
