Haidl Ian D, Marshall Jean S
Dalhousie Inflammation Group, Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada, B3H 4R2.
Methods Mol Biol. 2015;1220:179-201. doi: 10.1007/978-1-4939-1568-2_12.
Mast cells have been demonstrated to have critical roles in host defense against a number of types of pathogens. In order to better understand how mast cells participate in effective immune responses, it is important to evaluate their ability to respond directly to pathogens and their products. In the current chapter we provide a methodology to evaluate human mast cell responses to a number of bacterial and fungal pathogen products and to mammalian reovirus as a model of acute viral infection. These methods should provide key information necessary to aid in the effective design of experiments to evaluate human mast cell responses to a number of other organisms. However, it is important to carefully consider the biology of the mast cell subsets and pathogens involved and the optimal experimental conditions necessary to evaluate mediators of interest.
肥大细胞已被证明在宿主抵御多种病原体的过程中发挥关键作用。为了更好地理解肥大细胞如何参与有效的免疫反应，评估它们直接对病原体及其产物作出反应的能力很重要。在本章中，我们提供了一种方法，用于评估人类肥大细胞对多种细菌和真菌病原体产物以及作为急性病毒感染模型的哺乳动物呼肠孤病毒的反应。这些方法应提供关键信息，有助于有效设计实验，以评估人类肥大细胞对许多其他生物体的反应。然而，仔细考虑所涉及的肥大细胞亚群和病原体的生物学特性以及评估相关介质所需的最佳实验条件非常重要。
