使用荧光指示剂二乙酸二氨基荧光素（DAF-FM diacetate）测量肥大细胞中的一氧化氮。
Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate.
作者信息
St Laurent Chris D, Moon Tae Chul, Befus A Dean
机构信息
Pulmonary Research Group, Department of Medicine, University of Alberta, 116th Street & 87 Avenue, Edmonton, AB, Canada, T6G 2R3.
出版信息
Methods Mol Biol. 2015;1220:339-45. doi: 10.1007/978-1-4939-1568-2_21.
PMID:25388261
Abstract
The production of nitric oxide in mast cells has been difficult to measure due to the low amounts made by mast cells, as well as limitations in the specificity and sensitivity of the assays available. We present here a sensitive and specific 96-well plate-based method to directly measure NO using the cell-permeable fluorescent compound DAF-FM diacetate.
摘要
由于肥大细胞产生的一氧化氮量较少，以及现有检测方法在特异性和灵敏度方面存在局限性，因此很难测量肥大细胞中一氧化氮的产生量。我们在此介绍一种基于96孔板的灵敏且特异的方法，该方法使用可透过细胞的荧光化合物二乙酸二氨基荧光素（DAF-FM diacetate）直接测量一氧化氮。
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