Tijburg L B, Schuurmans E A, Geelen M J, van Golde L M
Biochim Biophys Acta. 1987 May 13;919(1):49-57. doi: 10.1016/0005-2760(87)90216-5.
The effect of vasopressin on the biosynthesis of phosphatidylcholines and phosphatidylethanolamines was investigated in freshly isolated rat hepatocytes in suspension. Treatment of hepatocytes with vasopressin inhibits the incorporation of [Me-14C]choline into phosphatidylcholines in a dose-dependent manner. The hormone does not affect the uptake, phosphorylation or oxidation of choline. Pulse-chase studies indicate that CTP:cholinephosphate cytidylyltransferase might be subject to hormonal regulation by vasopressin. In contrast with the inhibitory effect of vasopressin on the synthesis of phosphatidylcholines, this hormone stimulates the incorporation of [1,2-14C]ethanolamine into phosphatidylethanolamines in a dose-dependent manner. Pulse and pulse-chase studies with labelled ethanolamine show that the conversion of ethanolaminephosphate to CDPethanolamine as well as the formation of phosphatidylethanolamines from CDPethanolamine and diacylglycerol are enhanced. Determination of the effect of vasopressin on the activity of the enzymes of the synthesis de novo of phosphatidylethanolamines demonstrates an increase of the activity of ethanolaminephosphotransferase, probably as a result of the increased amount of diacylglycerol in vasopressin-treated cells.
在新鲜分离的悬浮大鼠肝细胞中研究了血管加压素对磷脂酰胆碱和磷脂酰乙醇胺生物合成的影响。用血管加压素处理肝细胞会以剂量依赖的方式抑制[甲基-14C]胆碱掺入磷脂酰胆碱。该激素不影响胆碱的摄取、磷酸化或氧化。脉冲追踪研究表明，CTP：胆碱磷酸胞苷转移酶可能受血管加压素的激素调节。与血管加压素对磷脂酰胆碱合成的抑制作用相反，该激素以剂量依赖的方式刺激[1,2-14C]乙醇胺掺入磷脂酰乙醇胺。用标记乙醇胺进行的脉冲和脉冲追踪研究表明，磷酸乙醇胺向CDP乙醇胺的转化以及由CDP乙醇胺和二酰基甘油形成磷脂酰乙醇胺均增强。测定血管加压素对磷脂酰乙醇胺从头合成酶活性的影响表明，乙醇胺磷酸转移酶的活性增加，这可能是血管加压素处理的细胞中二酰基甘油量增加的结果。
