胰高血糖素诱导培养的大鼠胎儿肝细胞中果糖2,6 -二磷酸和6 -磷酸果糖-2 -激酶的变化。
Glucagon-induced changes in fructose 2,6-bisphosphate and 6-phosphofructo-2-kinase in cultured rat foetal hepatocytes.
作者信息
Martin-Sanz P, Cascales M, Boscá L
机构信息
Instituto de Bioquimica del CSIC, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
出版信息
Biochem J. 1989 Feb 1;257(3):795-9. doi: 10.1042/bj2570795.
The sensitivity of 6-phosphofructo-2-kinase to glucagon and cyclic AMP was studied during the perinatal period. In liver homogenates from foetal and neonatal rats, incubation with cyclic AMP produced inactivation of 6-phosphofructo-2-kinase 3 h after birth. The maximal effect was obtained 12 h after birth. In primary cultures of hepatocytes from 22-day-old foetuses, glucogon induced an inhibition of 6-phosphofructo-2-kinase that required 45 min to reach the half-maximal effect. Cycloheximide prevented the glucagon-induced changes in this activity from cultured foetal hepatocytes. These results suggest that the adult form of 6-phosphofructo-2-kinase is rapidly induced after birth, probably by the hormonal changes that occur in this period.
在围产期研究了6-磷酸果糖-2-激酶对胰高血糖素和环磷酸腺苷（cAMP）的敏感性。在胎儿和新生大鼠的肝脏匀浆中，出生后3小时用环磷酸腺苷孵育会导致6-磷酸果糖-2-激酶失活。出生后12小时获得最大效应。在来自22日龄胎儿的肝细胞原代培养物中，胰高血糖素诱导6-磷酸果糖-2-激酶受到抑制，达到最大效应的一半需要45分钟。放线菌酮可防止胰高血糖素诱导的培养胎儿肝细胞中该酶活性的变化。这些结果表明，出生后可能由于这一时期发生的激素变化，迅速诱导出了6-磷酸果糖-2-激酶的成人形式。
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