Jung Su-Na, Kang Sang-Kee, Yeo Guen-Hye, Li Hai-Ying, Jiang Tao, Nah Jae-Woon, Bok Jin-Duck, Cho Chong-Su, Choi Yun-Jaie
Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Macromol Biosci. 2015 Mar;15(3):395-404. doi: 10.1002/mabi.201400352. Epub 2014 Nov 13.
The paper presents a novel dendritic cells (DC)-targeting peptide, TPAFRYS (TP) identified by phage display technique and conjugated to chitosan in order to develop an efficient DC-targeting vaccine delivery carrier. TP-conjugated chitosan nanoparticles (TPC-NPs) were prepared with ovalbumin (OVA) as a model vaccine by ionic gelation. Flow cytometry and immunocytochemistry studies demonstrated the higher targeting ability of TPC-NPs to DCs in compared to chitosan NPs. Moreover, TPC-NPs exhibited higher targeting specificity in DCs than macrophage and myoblasts. Furthermore, immunization of mice with OVA-loaded TPC-NPs enhanced OVA-specific serum IgG and IgG isotype antibodies production. Thus, DC-targeting strategy demonstrates a potential approach to enhance the effectiveness of vaccines.
本文介绍了一种通过噬菌体展示技术鉴定出的新型靶向树突状细胞（DC）的肽TPAFRYS（TP），并将其与壳聚糖偶联，以开发一种高效的靶向DC的疫苗递送载体。以卵清蛋白（OVA）为模型疫苗，通过离子凝胶法制备了TP偶联的壳聚糖纳米颗粒（TPC-NPs）。流式细胞术和免疫细胞化学研究表明，与壳聚糖纳米颗粒相比，TPC-NPs对DCs具有更高的靶向能力。此外，TPC-NPs在DCs中的靶向特异性高于巨噬细胞和成肌细胞。此外，用负载OVA的TPC-NPs免疫小鼠可增强OVA特异性血清IgG和IgG同种型抗体的产生。因此，靶向DC的策略证明了一种提高疫苗有效性的潜在方法。
