Chromosomal assignment of woodchuck hepatitis virus (WHV) DNA integration sites in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10).

作者信息

Mizuno Y, Murakami S, Matsushita F, Unoura M, Kobayashi K, Migita S, Hattori N, Ohno S

机构信息

First Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.

出版信息

Int J Cancer. 1989 Apr 15;43(4):652-7. doi: 10.1002/ijc.2910430419.

The chromosomal sites of woodchuck hepatitis virus (WHV) DNA integration were identified in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10) by the in situ hybridization technique using 3H-labelled WHV whole genome (WHV 2) as a probe. The G-banded chromosome spreads from WH257GE10 were identified and diagrammed schematically according to their band patterns. WHV DNA was integrated into 2 sites: 33 region of the long arm of chromosome 6 (6q33) and 31 region of the long arm of chromosome 8 (8q31). Chromosomal sites of WHV DNA integration were stable during successive passage periods analyzed in vitro.