Park Hye-Jung, Kim Moon-Moo
Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea.
Oncol Rep. 2015 Jan;33(1):433-8. doi: 10.3892/or.2014.3602. Epub 2014 Nov 12.
The aim of the present study was to examine the apoptotic effect of flavonoids in methanol extracts of Ginkgo biloba fallen leaves (MEGFL) on melanoma cells. Ginkgo biloba is a deciduous castle chaplain and its leaves include various types of flavonoids such as flavonol-O-glycosides. Ginkgo biloba is known to have therapeutic properties against a number of diseases such as cerebrovascular diseases, blood circulation disease and hypertension. In the present study MEGFL exhibited a higher cytotoxic effect on melanoma cells than Ginkgo biloba leaves (MEGL). It was also found that MEGFL induced apoptotic cell death which was characterized by DNA fragmentation. During the cell death process following treatment with MEGFL, the expression of a variety of death-associated proteins including p53, caspase-3, caspase-9, cytochrome c and Bax were analyzed in the cytosol of melanoma cells. MEGFL significantly increased the expression levels of caspase-3, caspase-9 and p53 in a dose-dependent manner. Our results indicate that MEGFL induced apoptotic cell death by increasing the expression of cell death-associated proteins in melanoma cells.
本研究的目的是检测银杏落叶甲醇提取物（MEGFL）中的黄酮类化合物对黑色素瘤细胞的凋亡作用。银杏是一种落叶乔木，其叶子含有多种黄酮类化合物，如黄酮醇 - O - 糖苷。众所周知，银杏对多种疾病具有治疗作用，如脑血管疾病、血液循环疾病和高血压。在本研究中，MEGFL对黑色素瘤细胞的细胞毒性作用比银杏叶（MEGL）更高。还发现MEGFL诱导了以DNA片段化为特征的凋亡性细胞死亡。在用MEGFL处理后的细胞死亡过程中，分析了黑色素瘤细胞胞质溶胶中包括p53、半胱天冬酶 - 3、半胱天冬酶 - 9、细胞色素c和Bax在内的多种死亡相关蛋白的表达。MEGFL以剂量依赖性方式显著增加了半胱天冬酶 - 3、半胱天冬酶 - 9和p53的表达水平。我们的结果表明，MEGFL通过增加黑色素瘤细胞中细胞死亡相关蛋白的表达诱导凋亡性细胞死亡。
