Tonge P, Moore G R, Wharton C W
Department of Biochemistry, University of Birmingham, U.K.
Biochem J. 1989 Mar 1;258(2):599-605. doi: 10.1042/bj2580599.
The alkaline transitions of tuna and horse ferricytochromes c and the trifluoroacetyl-lysine derivative of horse ferricytochrome c have been studied by Fourier-transform (FT) i.r. spectroscopy. The spectral perturbations resulting from the transition have been interpreted by reference to FT i.r. data on simple carboxylic-acid-containing compounds and a bacterial cytochrome c551 in which a haem propionate ionizes without causing a significant conformational change. The analysis strongly suggests that ionization of a haem propionate of mitochondrial cytochrome c triggers the alkaline conformation change.
通过傅里叶变换红外光谱法研究了金枪鱼和马的高铁细胞色素c的碱性转变以及马高铁细胞色素c的三氟乙酰赖氨酸衍生物。参照含简单羧酸化合物和细菌细胞色素c551的傅里叶变换红外光谱数据对转变产生的光谱扰动进行了解释,在细菌细胞色素c551中,血红素丙酸酯发生电离而不引起显著的构象变化。分析结果有力地表明,线粒体细胞色素c的血红素丙酸酯的电离触发了碱性构象变化。