Eley C G, Moore G R, Williams R J, Neupert W, Boon P J, Brinkhof H H, Nivard R J, Tesser G I
Biochem J. 1982 Jul 1;205(1):153-65. doi: 10.1042/bj2050153.
The tertiary structures of horse, tuna, Neurospora crassa, horse [Hse65,Leu67]- and horse [Hse65,Leu74]-cytochromes c were studied with high-resolution 1H n.m.r. spectroscopy. The amino acid sequences of these proteins differ at position 46, which is occupied by phenylalanine in the horse proteins but by tyrosine in the remaining two, and at positions 67, 74 and 97, which are all occupied by tyrosine residues in horse and tuna cytochrome c but in the other proteins are substituted by phenylalanine or leucine, though there is only one such substitution per protein. The various aromatic-amino-acid substitutions do not seriously affect the protein structure.
利用高分辨率的¹H核磁共振光谱研究了马、金枪鱼、粗糙脉孢菌、马[Hse65,Leu67] - 和马[Hse65,Leu74] - 细胞色素c的三级结构。这些蛋白质的氨基酸序列在第46位不同，马的蛋白质中该位置为苯丙氨酸，其余两种为酪氨酸；在第67、74和97位也不同，马和金枪鱼细胞色素c中这些位置均为酪氨酸残基，而在其他蛋白质中被苯丙氨酸或亮氨酸取代，不过每种蛋白质只有一处这样的取代。各种芳香族氨基酸取代对蛋白质结构没有严重影响。
