马心脏细胞色素c碱性转变过程中配体交换过程的鉴定
Identification of the ligand-exchange process in the alkaline transition of horse heart cytochrome c.
作者信息
Gadsby P M, Peterson J, Foote N, Greenwood C, Thomson A J
机构信息
School of Chemical Sciences, University of East Anglia, Norwich, U.K.
出版信息
Biochem J. 1987 Aug 15;246(1):43-54. doi: 10.1042/bj2460043.
Abstract
Magnetic-circular-dichroism (m.c.d.) spectra over the wavelength range 300-2000 nm at room temperature and at 4.2K of horse heart cytochrome c are reported at a series of pH values between 7.8 and 11.0, encompassing the alkaline transition. The effect of glassing agents on the e.p.r. spectrum at various pH values is also reported. Comparison of these results with spectra obtained for the n-butylamine adduct of soybean leghaemoglobin support the hypothesis that lysine is the sixth ligand in the alkaline form of horse heart cytochrome c. The m.c.d. and e.p.r. spectra of horse heart cytochrome c in the presence of 1-methylimidazole have also been examined. These studies strongly suggest that histidine-18, the proximal ligand of the haem, is the ionizing group that triggers the alkaline transition. Low-temperature m.c.d. and e.p.r. spectra are also reported for Pseudomonas aeruginosa cytochrome c551. It is shown that no ligand exchange takes place at the haem in this species over the pH range 6.0-11.3.
摘要
本文报道了马心细胞色素c在室温及4.2K下，波长范围为300 - 2000nm、pH值在7.8至11.0之间（涵盖碱性转变）一系列条件下的磁圆二色性（m.c.d.）光谱。还报道了在不同pH值下玻璃化剂对电子顺磁共振（e.p.r.）光谱的影响。将这些结果与大豆豆血红蛋白正丁胺加合物的光谱进行比较，支持了赖氨酸是马心细胞色素c碱性形式中第六个配体的假说。还研究了在1 - 甲基咪唑存在下马心细胞色素c的m.c.d.和e.p.r.光谱。这些研究有力地表明，血红素的近端配体组氨酸 - 18是引发碱性转变的电离基团。本文还报道了铜绿假单胞菌细胞色素c551的低温m.c.d.和e.p.r.光谱。结果表明，在该物种中，pH值范围为6.0 - 11.3时，血红素处未发生配体交换。
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