Taguchi J, Kuriyama T, Ohmori Y, Kuriyama K
Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
Brain Res. 1989 Apr 3;483(2):395-401. doi: 10.1016/0006-8993(89)90187-x.
The distribution of the gamma-aminobutyric acid (GABA)A receptor/benzodiazepine receptor/Cl- channel complex in the rat brain was examined immunohistochemically using the specific antibody against purified GABAA receptor complex. The immunization of white albino rabbit with purified GABAA receptor complex resulted in the formation of specific antibody as indicated by the immunoprecipitation test. Immunohistochemical examinations using the antiserum on rat brain slices by the peroxidase-antiperoxidase method revealed the presence of the following immunoreactive sites which coincided with a previous report using antibody against L-glutamic acid decarboxylase; ventromedial nucleus of hypothalamus, red nucleus, globus pallidus, zona compacta and zona reticulata of substantia nigra, layers of Purkinje cells and granular cells of cerebellum, layers III-V of cerebral cortex and stratum radiatum of hippocampus. These results strongly suggest that immunohistochemical application of the antibody against the purified GABAA receptor complex is a useful tool for identifying GABAergic neurons having GABAA receptor complex-mediated synapses.
使用针对纯化的γ-氨基丁酸（GABA）A受体复合物的特异性抗体，通过免疫组织化学方法检测了大鼠脑中GABA A受体/苯二氮䓬受体/氯离子通道复合物的分布。用纯化的GABA A受体复合物对白化家兔进行免疫，免疫沉淀试验表明产生了特异性抗体。用过氧化物酶-抗过氧化物酶法，使用抗血清对大鼠脑切片进行免疫组织化学检查，发现了以下免疫反应位点，这些位点与先前使用抗L-谷氨酸脱羧酶抗体的报告一致；下丘脑腹内侧核、红核、苍白球、黑质致密部和网状部、小脑浦肯野细胞层和颗粒细胞层、大脑皮层III-V层和海马辐射层。这些结果有力地表明，针对纯化的GABA A受体复合物的抗体的免疫组织化学应用是识别具有GABA A受体复合物介导突触的GABA能神经元的有用工具。
